pCDCAR1 MUC1 h(28γ), T(CAR-T-2-M322-2G)

Datasheet

The vector of anti-MUC1 chimeric antigen receptor (CAR) is constructed for the engineering of T cells to target human MUC1. The T cells are genetically modified through transduction with a lentiviral vector expressing scFv of anti-MUC1 antibody linked to CD28 transmembrane domain/ endodomain and FcεRIγ signaling domains. And the vector product was designed for the treatment of MUC1-expressing tumors .

Details

Target

MUC1

Target Species

Human

Targeting Cell Type

T cell

Targeting Diseases

MUC1-expressing tumors

Generation

Second

Vector Name

pCDCAR1

Vector Length

8kb

Vector Type

Lentiviral

Receptor Construction

scFv-CD28-FcεRIγ

Discription of Signaling Cassetes

CD28
CD28 (Cluster of Differentiation 28) is one of the proteins expressed on T cells that provide co-stimulatorysignals required for T cell activation and survival. CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2) proteins which are expressed on antigen-presenting cells(APC). CD28 modulates the primary TCR/CD3ζ signal in a different fashion than the late costimulatory elements OX40 and 4-1BB. CD28 enhances the expression of downstream regulators that impact on T-cell proliferation, death, differentiation, and effector functions. CAR+ T cells containing the CD28 endodomain showed strikingly enhanced sustained T cell activation, growth, survival. And CD28 results in a brightly expressed, stable receptor as the transmembrane domain. Including CD28 costimulatory domains in CARs led to enhanced anti-malignancy efficacy.
FcεRIγ
The high-affinity IgE receptor, also known as FcεRI, or Fc epsilon RI, is the high-affinity receptor for the Fc region of immunoglobulin E (IgE). FcεRI is a key molecule involved in allergic reactions. It is a tetramer composed of 1alpha, 1 beta, and 2 gamma chains. The gamma chains are also subunits of other Fc receptors. The scFv-based CARs engineered to contain a signaling domain from FcεRIγ have been shown to deliver a potent signal for T cell activation and function.

Antibody Clone

HMFG2

Antibody Host

Mouse

Target

Gene Name

mucin 1, cell surface associated

Official Symbol

MUC1

Gene ID

4582

Synonyms

MUC1; mucin 1, cell surface associated; EMA; MCD; PEM; PUM; KL-6; MAM6; MCKD; PEMT; CD227; H23AG; MCKD1; MUC-1; ADMCKD; ADMCKD1; CA 15-3; MUC-1/X; MUC1/ZD; MUC-1/SEC; MUC1-expressing tumors; scFv; FcεRIγ; Chimeric antigen receptor; T cell; Retrovirus; CARs; Lentivirus; chimeric T cell receptors; chimeric immunoreceptors; chimeric antigen receptors; HMFG2

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

Related Products

Cat#	Description
CAR-T-1-M322-G	Anti-MUC1 scFv h(FcεRIγ) CART, pCDCAR1
CAR-T-3-M303-2BZ	Anti-MUC1 scFv h(CD28-41BB-CD3ζ) CART, pCDCAR1
CAR-T-1-M553-2	Anti-MUC1 VHH h(CD28) CAR, [T]
VP-CAR-LC370	Lenti-MUC1 CAR (scFv-CD4-FcεRIγ, SM3) Viral Particle
CAR-T-2-M553-4G	Anti-MUC1 VHH h(CD4-FcεRIγ) CAR, [T]
VP-CAR-LC377	Lenti-MUC1 CAR (scFv-CD28, HMFG2) Viral Particle
VP-CAR-LC387	Lenti-MUC1 CAR (scFv-CD28-41BB-CD3ζ, HMFG2) Viral Particle
VP-CAR-LC367	Lenti-MUC1 CAR (scFv-CD3ζ, SM3) Viral Particle
CAR-LC251	Anti-MUC1 h(CD28-41BB-CD3ζ) CAR, pCDCAR1
CAR-T-1-M322-2	Anti-MUC1 scFv h(CD28) CART, pCDCAR1

CarcinomaOthers Kits for CARs/TCRs Development

Online Inquiry

CONTACT US
45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-381-2994
Europe: 44-207-097-1828
Fax: 1-631-207-8356
Email:

pCDCAR1 MUC1 h(28γ), T(CAR-T-2-M322-2G)

Details

Target

Related Products

Online Inquiry

CONTACT US

Distributors

FOLLOW US ON