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Equipped with state-of-the-art facilities and experienced immunology experts, Creative Biolabs can offer complete genotyping services for our global clients. With advanced technology platforms and extensive expertise, we are ready to deliver accurate, flexible, and cost-effective SLA genotyping service solutions.
The swine model has been an important contributor to the study, particularly for allo- and xeno-transplantation studies using SLA-defined pigs. Molecular characterization of swine leukocyte antigen (SLA) genes is of great significance in illuminating the immune responses to swine-donor and human-recipient in xenotransplantation.
The SLA gene complex is located on chromosome 7 and spans the centromere. The complex is the most gene-dense region in the porcine genome, and it includes three major gene classes.
The SLA class I antigens includes seven classical class I genes and three non-classical class I genes. The constitutively expressed classical SLA class I genes are SLA-1, SLA-2, and SLA-3, while the rest are pseudogenes. The non-classical class I genes are SLA-6, SLA-7, and SLA-8, and are located at the centromeric end of the class I region.
There are many genes encoding the expressed SLA class II antigens, which include the a- and b-chain genes for the SLA-DR, - DQ, -DM, and -DO proteins. The major expressed SLA class II genes include DRB1, DQA, DQB1, DOB1, DMB, DMA, and DOA. While DRB2, DRB3, DRB4, DRB5, DQB2, DOB2, and wDYB belong to the SLA class II pseudogenes.
The SLA class III region is centromeric and joined to the contiguous class I region physically. Which includes many important genes in the immune defense system, such as the tumor necrosis factor (TNF) gene families and components of the complement cascade (C2, C4A, and CFB).
Fig.1 Comparative genomic organization of the human and swine major histocompatibility complex (MHC) class I region.
Fig.2 Comparative genomic organization of the human and swine major histocompatibility complex (MHC) class II region.
To identify the complexity of SLA polymorphism, Creative Biolabs developed several methods which are based on PCR-sequence-specific primers (SSP-PCR), PCR-restriction fragment length polymorphism (RFLP-PCR), or microsatellite (MS) markers. Those methods allow cost-effective, precise, and fast generation of results.
In addition, we developed a user-friendly computational application that interprets the raw data and reliably exports the final genotyping results in spreadsheet file format. The combination of a reliable method that requires low amount of DNA with an automated interpretation of results allows scaling the SLA genotyping in large cohorts with reduced turnaround time.
For more details of our SLA genotyping service, please visit our web for more information. To request other genotyping services, please reach out to our scientists to learn how we can be involved in your project.
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