Equipped with world-leading phage display platform and professional scientific staff, Creative Biolabs has developed a variety of biopanning strategies for the identification of abzymes. Our scientists are pleased to tailor the most appropriate strategies to screen the interested libraries of our global customers.
Catalytic antibody (abzyme) is a type of monoclonal antibody with catalytic activity. Natural abzymes can be found not only in healthy humans and but also in patients with autoimmune diseases such as systemic lupus erythematosus. Anti-vasoactive intestinal peptide autoantibodies are found in normal humans, where they can bind to and hydrolyze DNA. Abzymes are usually generated by the immunization of animals with synthetic haptens. So far, this technique has proven most successful for the elicitation of abzymes that approach the catalytic efficiencies of natural enzymes. Phage display is a powerful and widely used technique in the biological and medical research fields. The rapid recovery of specific antibody candidates by robust screening of a variety of target antigens is one of the most successful uses of this technique today. Using a suicide substrate of β-lactamase as a target, catalytic antibodies with β-lactamase activity have been identified by phage display technology. Studying abzymes can provide important insights into enzyme reaction mechanisms and the immune system itself.
Based on our high-throughput phage display platform, Creative Biolabs has developed a variety of strategies for the identification of abzymes with high specificity and affinity. Several rounds of biopanning against given target are performed to enrich the pool of high-affinity binders. A sufficient number of the selected phages is screened for the identification of positive clones, exhibiting high affinity toward the target. Combined with our advanced Magic™ platform, abzymes in the enriched sublibrary can be identified efficiently and effectively.
Key advantages of abzymes discovery service on our phage display platform including but not limited to:
Through our advanced system, clients could accelerate their research with the most comprehensive view of antibody and with reducing project costs and timelines. Creative Biolabs is pleased to share our cutting-edge technology and extensive expertise in identifying abzymes with high specificity and affinity to facilitate our clients’ related project development. We can offer high-quality custom services by adjusting protocols to meet every unique requirement. Please feel free to inquire us for more information and a detailed quotation.