Introduction of CHRNA9
CHRNA9, a gene encoding the neuronal acetylcholine receptor subunit alpha-9, is localized to chromosome 4p14 and contains five exons, separated by four introns. Exons 1-5 are 78, 145,154, 532 and 877 bases, respectively. CHRNA9 protein is a plasma membrane protein of divalent cation channels and is found in human epidermal keratinocytes. To date, these particular receptors are found predominantly in the inner ear and specifically on the surface of type II hair cells. CHRNA9 connected with CHRNA10 form homo- or heterooligomeric channels. The native outer hair cell receptor may be composed of CHRNA9-CHRNA10 heterooligomers.
|Basic Information of CHRNA9|
|Protein Name||Neuronal acetylcholine receptor subunit alpha-9|
|Aliases||Nicotinic acetylcholine receptor subunit alpha-9 (NACHR alpha-9)|
|Organism||Homo sapiens (Human)|
Function of CHRNA9 Membrane Protein
Neuronal acetylcholine receptor subunit alpha-9 may play a role in the modulation of auditory stimuli. Conformation resulting from change agonist binding induces a leads to the opening of an ion-conducting channel across the plasma membrane. The channel is permeable to a range of divalent cations including calcium. The divalent cations influx may activate a potassium current which hyperpolarizes the cell membrane. In the ear, this may lead to a reduction in basilar membrane motion, altering the activity of auditory nerve fibers and reducing the range of dynamic hearing. This may also protect against acoustic trauma and regulate keratinocyte adhesion. Additionally, previous studies have demonstrated overexpression and activation of CHRNA9 protein during human breast epithelial cell tumorigenesis, while inhibition of CHRNA9 protein signaling shows antitumor effects on human breast cancer cells.
Fig.1 Schematic summary of the (phospho-) cholinergic inhibition of ATP-induced inflammasome activation in monocytes. Endogenous ligands of CHRNA7, CHRNA9, and/or CHRNA10, such as ACh, choline, or PC, inhibit ATP-dependent release of IL-1β (Hecker, 2015)
Application of CHRNA9 Membrane Protein in Literature
This article uncovers a novel role for the α9α10-nAChR in mounting a normal stress response and in the regulation of affective- and reward-related behaviour, and suggests that pursuing the receptor for clinical treatments may not be as straightforward as has been suggested.
This article confirms that hypothermia is a biological correlate of a nausea-like state in animals using knockout (KO) mice lacking alpha-9 nicotinic acetylcholine receptors as a wild-type (WT) control.
The CHRNA9 rs6819385 SNP was significantly related to an increased risk of NSCLC in Chinese population.
This article supports that CHRNA9 gene and smoking exposure have correlation on the risk of breast cancer development.
This article reveals that α9-nAChR expression is important to mediate TNBC metastasis during cancer development and may potentially function as a biomarker for targeted therapy in clinical investigations.
CHRNA9 preparation options
Membrane protein studies have developed greatly over the past few years. Based on our versatile Magic™ membrane protein production platform, we could offer a series of membrane protein preparation services for worldwide customers in reconstitution forms as well as multiple active formats. Aided by our versatile Magic™ anti-membrane protein antibody discovery platform, we also provide customized anti-CHRNA9 antibody development services.
During the past years, Creative Biolabs has successfully generated many functional membrane proteins for our global customers. We are happy to accelerate the development of our clients’ programs with our one-stop, custom-oriented service. For more detailed information, please feel free to contact us.