Citrullination Specific Antibody Discovery Service

Background Antibody Types Discovery Strategy Types of PTM Why Choose Us? FAQs

Is detecting citrullinated proteins, which functions as a vital post-translational modification, problematic for you since it is implicated in various diseases? Our Citrullination Specific Antibody Discovery Services at Creative Biolabs deliver highly specific antibodies through our expert team who operate advanced technology. We offer specialized solutions to meet your unique research needs and provide dependable and repeatable results.

Background

The scientific community defines citrullination as deimination which changes arginine residues into citrulline during post-translational modification. The set of enzymes peptidylarginine deiminases (PADs) facilitates the biochemical transformation. Protein citrullination leads to structural and functional alterations that impact both normal bodily functions and disease development.

Fig. 1 ACPA bone destruction structure. (OA Literature) Fig.1 ACPA bone destruction.¹

Studies show that citrullination takes place in various diseases, including autoimmune conditions such as rheumatoid arthritis (RA), along with cancer and neurodegenerative disorders. Anti-citrullinated protein antibodies (ACPAs) serve as important disease markers in rheumatoid arthritis (RA) patients who frequently display these antibodies. Studies demonstrate that nanomaterials have the potential to trigger protein citrullination, which could lead to disease formation. Research into citrullinated proteins reveals fundamental disease mechanisms and facilitates the creation of diagnostic techniques and therapeutic goals. Access to high-quality antibodies that target citrullinated proteins requires specialized service offerings. Creative Biolabs provides solutions tailored to specific topics along with fundamental problem-solving capacities.

Antibody Types

Creative Biolabs provides full-service solutions for developing both monoclonal and polyclonal antibodies. Production of antibodies takes place using rabbit, mouse, and sheep as host organisms. Our citrullination-specific antibody offerings include:

Site-Specific Anti-Citrullination Antibodies: These antibodies identify citrullination at specific amino acid residues within a protein. The antibodies function as accurate instruments to study citrullination effects at specific protein sites and enable the detection of proteins that experienced citrullination.
Pan Anti-Citrullination Antibodies: These antibodies recognize citrullinated proteins without relying on the specific amino acid sequence involved. These antibodies serve to quantify total citrullination within proteins and identify proteins that have been citrullinated.

Discovery Strategy

Creative Biolabs uses three fundamental approaches to create antibodies that target citrullination.

Monospecific Anti-Citrullination Polyclonal Antibody Production: During polyclonal antibody production, host animals are given a citrullinated antigen which has been specially designed for this purpose. Our exclusive techniques boost antibody specificity while minimizing cross-reactivity with normal proteins.

Fig. 2 General process of monospecific anti-citrullination polyclonal antibody production. (Creative Biolabs Original)

Phage Display Strategy for Anti-Citrullination Monoclonal Antibody Discovery: The phage display technique functions as an effective system to produce monoclonal antibodies. Our research employs bacteriophage-displayed antibody fragment libraries to select clones that show specific binding to citrullinated antigens. This methodology enables swift detection of antibodies possessing strong binding affinity.

Fig. 3 General process of phage display for anti-citrullination monoclonal antibody discovery. (Creative Biolabs Original)

Hybridoma Strategy for Anti-Citrullination Monoclonal Antibody Discovery: Hybridoma technology produces immortal hybridomas from a fusion of antibody-producing B cells and myeloma cells, which generate monoclonal antibodies. Our sophisticated screening technology enables us to detect hybridomas that produce antibodies targeting only citrullinated proteins.

Fig. 4 General process of hybridoma for anti-citrullination monoclonal antibody discovery. (Creative Biolabs Original)

Types of PTM

We provide Citrullination specific antibodies and antibodies targeting a variety of PTMs, as shown in the table below.

Why Choose Us?

Creative Biolabs stands out as a premier antibody development service provider with profound expertise in crafting precise antibodies for diverse targets, including post-translational modifications such as citrullination. Our Citrullination Specific Antibody Discovery Services deliver multiple significant benefits.

High Specificity: Through proprietary technologies such as our High-Affi™ technology, we create antibodies with superior specificity to target citrullinated proteins. Our methodology reduces the possibility of inaccurate positive findings while maintaining your data's dependability.
Customizable Solutions: We recognize that every research project demands specific requirements. Our service options provide flexibility so we can customize protocols to accommodate your specific requirements, such as antigen design, as well as host animal selection and antibody purification strategies.
Comprehensive Service Portfolio: We provide citrullination-specific antibody services, which sit under a larger selection of PTM-specific antibody production services, including anti-phosphorylation, anti-ubiquitination, and anti-acetylation. You can secure a full solution for your protein modification research requirements through our services.
Expert Team: Our scientists' extensive experience translates into deep expertise within the fields of antibody development and the biology of citrullination. Our experienced team delivers continuous expert consultation and support at every stage to guarantee your project's success.
Proven Track Record: Creative Biolabs demonstrates established expertise in producing top-tier antibodies that serve a wide range of research applications. Research publications demonstrate the effectiveness and dependability of our provided services.

FAQs

Q: How can I determine if a citrullination-specific antibody is appropriate for my research?

A: Our antibodies become indispensable tools when your study focuses on protein citrullination processes or analyzes their disease connections and treatment responses. These antibodies deliver both specificity and sensitivity, which enable precise detection and measurement of citrullinated proteins. Send us a message and we will assist you in finding the ideal antibody for your application needs.

Q: What sample types can your citrullination-specific antibodies be used for?

A: Our antibodies function with diverse sample types such as cell lysates, tissue extracts, and serum. Your particular sample type will determine which antibody and protocol work best. Our team is available to help you with sample preparation and assay optimization guidance when you contact us.

Q: Is it possible to obtain an antibody that targets citrullination at a precise location on my selected protein?

A: Our services allow you to produce antibodies that target citrullination at specific protein sites. Scientists have developed these antibodies to detect citrullination at specific amino acid residues, which offers precise instruments for your research projects. Contact us to discuss your specific requirements.

Q: What is the performance comparison between citrullination-targeting antibodies from your company and those found in different commercial providers?

A: Advanced technologies and rigorous quality control measures enable Creative Biolabs to manufacture antibodies with exceptional specificity and high affinity. Researchers find that our antibodies generate consistent results, which allow for successful replication across multiple experiments, better than most commercial antibodies. Connect with us to access all necessary product details along with performance results.

Q: Which customer support services will you provide following delivery of the antibodies?

A: We ensure our customers receive ongoing support throughout their experience. A team of professional experts stands prepared to respond to your inquiries and provide technical assistance with troubleshooting solutions for your challenges. We aim to achieve full customer satisfaction by providing excellent products alongside superior services. Please inquire for more details.

Creative Biolabs delivers superior citrullination-specific antibodies and extensive support solutions to expedite your scientific investigations. Reach out to our team to obtain more information and initiate a discussion regarding your project. Do not hesitate to contact us if you need any help.

Reference

Liu, Jiaxi, et al. "Anti-citrullinated protein antibody generation, pathogenesis, clinical application, and prospects." Frontiers in Medicine 8 (2022): 802934. Distributed under Open Access license CC BY 4.0, without modification.

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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