Introduction of CNNM1
CNNM1 is encoded by the CNNMI gene which is located at 10q24.2. It belongs to the ancient conserved domain protein (ACDP) family which is a recently identified group of homologous mammalian proteins. Subcellular localization analysis suggests that CNNM1 is targeted to the cell membrane and it is a multi-pass membrane protein. The molecular weight of CNNMI is 104,351 Da in human. The CNNM1 protein has been identified in both humans and mice and homologues identified in bacteria, yeast and invertebrates.
|Basic Information of CNNM1|
|Protein Name||Metal transporter CNNM1|
|Aliases||Ancient conserved domain-containing protein 1, Cyclin-M1|
|Organism||Homo sapiens (Human)|
|Transmembrane Times||Multi-pass membrane|
Function of CNNM1 Membrane Protein
CNNM1 is a high-affinity copper binding protein which is able to bind copper at nanomolar concentrations. On the one hand, the promoter of CNNM1 contains metal response, on the other hand, the cellular expression of ACDP-1 alters cellular retention of copper. However, CNNMI expression does not protect against the toxic effects of copper or other metals. Beyond that, CNNM1 has been proved to take part in ion transport which means directed movement of charged atoms or small charged molecules. What’s more, CNNM1 is a putative cell cycle regulator expressed mainly in testis and brain, while the other variants are expressed ubiquitously in both embryonic and adult tissues. CNNM1 expression appeared to be restricted to c-KIT and OCT3/4-positive cells in the testis, indicating them to be early spermatogonial cell. It is associated with stemness and self-renewal. CNNM1 is also differentially displayed in the spermatozoa from several of the infertile men.
Fig.1 Predicted three-dimensional structure of CNNM1 (Wang, 2003).
Application of CNNM1 Membrane Protein in Literature
This article shows that CNNM1 is associated with stemness and self-renewal, and its down-regulation triggers differentiation in spermatogonial cells in mouse.
Authors of this article focus on pregnancy problem. Since CNNM1 is associated with testis, it has also been studied. They show that Cnnm1 is expressed in the testes of mice from neonatal to adult stages with relatively higher levels in neonates. CNNM1 expression appeared to be restricted to c-KIT and OCT3/4-positive cells in the testis and so on.
Authors in this group reveal that ACDP-1 is a high afﬁnity copper binding protein. The cellular expression of ACDP-1 alters cellular retention of copper. However, cellular expression of ACDP-1 does not alter cellular resistance to the toxicity of copper or other metals.
Authors in this article clone four novel human genes that encode the ancient conserved domain proteins. One of them is CNNM1. They also reveal some characterization of the CNNM genes and their encoded protein products.
CNNM1 Preparation Options
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