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CNNM1 Membrane Protein Introduction

Introduction of CNNM1

CNNM1 is encoded by the CNNMI gene which is located at 10q24.2. It belongs to the ancient conserved domain protein (ACDP) family which is a recently identified group of homologous mammalian proteins. Subcellular localization analysis suggests that CNNM1 is targeted to the cell membrane and it is a multi-pass membrane protein. The molecular weight of CNNMI is 104,351 Da in human. The CNNM1 protein has been identified in both humans and mice and homologues identified in bacteria, yeast and invertebrates.

Basic Information of CNNM1
Protein Name Metal transporter CNNM1
Gene Name CNNM1
Aliases Ancient conserved domain-containing protein 1, Cyclin-M1
Organism Homo sapiens (Human)
UniProt ID Q9NRU3
Transmembrane Times Multi-pass membrane
Length (aa) 951
Sequence MAAAAAAAAAVGVRLRDCCSRGAVLLLFFSLSPRPPAAAAWLLGLRPEDTAGGRVSLEGGTLRAAEGTSFLLRVYFQPGPPATAAPVPSPTLNSGENGTGDWAPRLVFIEEPPGGGGVAPSAVPTRPPGPQRCREQSDWASDVEVLGPLRPGGVAGSALVQVRVRELRKGEAERGGAGGGGKLFSLCAWDGRAWHHHGAAGGFLLRVRPRLYGPGGDLLPPAWLRALGALLLLALSALFSGLRLSLLSLDPVELRVLRNSGSAAEQEQARRVQAVRGRGTHLLCTLLLGQAGANAALAGWLYTSLPPGFGGTGEDYSEEGIHFPWLPALVCTGAVFLGAEICPYSVCSRHGLAIASHSVCLTRLLMAAAFPVCYPLGRLLDWALRQEISTFYTREKLLETLRAADPYSDLVKEELNIIQGALELRTKVVEEVLTPLGDCFMLRSDAVLDFATVSEILRSGYTRIPVYEGDQRHNIVDILFVKDLAFVDPDDCTPLLTVTRFYNRPLHCVFNDTRLDTVLEEFKKGKSHLAIVQRVNNEGEGDPFYEVMGIVTLEDIIEEIIKSEILDETDLYTDNRKKQRVPQRERKRHDFSLFKLSDTEMRVKISPQLLLATHRFMATEVEPFKSLYLSEKILLRLLKHPNVIQELKFDEKNKKAPEHYLYQRNRPVDYFVLLLQGKVEVEVGKEGLRFENGAFTYYGVPAIMTTACSDNDVRKVGSLAGSSVFLNRSPSRCSGLNRSESPNRERSDFGGSNTQLYSSSNNLYMPDYSVHILSDVQFVKITRQQYQNALTACHMDSSPQSPDMEAFTDGDSTKAPTTRGTPQTPKDDPAITLLNNRNSLPCSRSDGLRSPSEVVYLRMEELAFTQEEMTDFEEHSTQQLTLSPAAVPTRAASDSECCNINLDTETSPCSSDFEENVGKKLLRTLSGQKRKRSPEGERTSEDNSNLTPLIT

Function of CNNM1 Membrane Protein

CNNM1 is a high-affinity copper binding protein which is able to bind copper at nanomolar concentrations. On the one hand, the promoter of CNNM1 contains metal response, on the other hand, the cellular expression of ACDP-1 alters cellular retention of copper. However, CNNMI expression does not protect against the toxic effects of copper or other metals. Beyond that, CNNM1 has been proved to take part in ion transport which means directed movement of charged atoms or small charged molecules. What’s more, CNNM1 is a putative cell cycle regulator expressed mainly in testis and brain, while the other variants are expressed ubiquitously in both embryonic and adult tissues. CNNM1 expression appeared to be restricted to c-KIT and OCT3/4-positive cells in the testis, indicating them to be early spermatogonial cell. It is associated with stemness and self-renewal. CNNM1 is also differentially displayed in the spermatozoa from several of the infertile men.

CNNM1 Membrane Protein IntroductionFig.1 Predicted three-dimensional structure of CNNM1 (Wang, 2003).

Application of CNNM1 Membrane Protein in Literature

  1. Chandran U., et al. Expression of Cnnm1 and Its Association with Stemness, Cell Cycle, and Differentiation in Spermatogenic Cells in Mouse Testis1. Biology of Reproduction. 2016, 95(1). PubMed ID: 27251091

    This article shows that CNNM1 is associated with stemness and self-renewal, and its down-regulation triggers differentiation in spermatogonial cells in mouse.

  2. Kumar P.G., et al. Identification of molecular markers of male factor infertility using sperm proteome profiling. ISSRF Newsletter. 2016, (19). 16-21.

    Authors of this article focus on pregnancy problem. Since CNNM1 is associated with testis, it has also been studied. They show that Cnnm1 is expressed in the testes of mice from neonatal to adult stages with relatively higher levels in neonates. CNNM1 expression appeared to be restricted to c-KIT and OCT3/4-positive cells in the testis and so on.

  3. Alderton A., et al. Ancient conserved domain protein-1 binds copper and modifies its retention in cells. Journal of Neurochemistry. 2007, 103(1):312-321. PubMed ID: 17608643

    Authors in this group reveal that ACDP-1 is a high affinity copper binding protein. The cellular expression of ACDP-1 alters cellular retention of copper. However, cellular expression of ACDP-1 does not alter cellular resistance to the toxicity of copper or other metals.

  4. Wang C.Y., et al. Molecular cloning and characterization of a novel gene family of four ancient conserved domain proteins (ACDP). Gene. 2003, 306(6):37. PubMed ID: 12657465

    Authors in this article clone four novel human genes that encode the ancient conserved domain proteins. One of them is CNNM1. They also reveal some characterization of the CNNM genes and their encoded protein products.

CNNM1 Preparation Options

To obtain the soluble and functional target protein, the versatile Magic™ membrane protein production platform in Creative Biolabs enables many flexible options, from which you can always find a better match for your particular project. Aided by our versatile Magic™ anti-membrane protein antibody discovery platform, we also provide customized anti-CALHM1 antibody development services.


As a forward-looking research institute as well as a leading customer service provider in the field of membrane protein, Creative Biolabs has won good reputation among our worldwide customers for successfully accomplishing numerous challenging projects including generation of many functional membrane proteins. Please feel free to contact us for more information.

Reference

  1. Wang C. Y., et al. (2003). Molecular cloning and characterization of a novel gene family of four ancient conserved domain proteins (acdp). Gene. 306(6), 37.

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