This product is an aptamer which binds to the Thyroxine with an affinity of <50 µM.
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| Target Category | Small Organic |
| Target | Thyroxine |
| Target Overview | Thyroxine is the main hormone secreted into the bloodstream by the thyroid gland. It plays vital roles in digestion, heart and muscle function, brain development and maintenance of bones. Thyrotropin-releasing hormone Triiodothyronine Glossary All Hormones Resources for Hormones. |
| Chemical Modification | RNA |
| Length | 40-60 nt |
| Affinity (Kd) | <50 µM |
| Binding Conditions/Buffer | Loading buffer: 10mM Tris-HCl (pH 7.5), 500 mM NaCl, and 1 mM MgCl2. |
| GC Content | 0.6591 |
| Molecular Weight | 14313.6 g/mole |
| Extinction Coefficient | 424000 L/(mole·cm) |
| nmoles/OD260 | 2.36 |
An essential tool in our labExcellentIts specificity is impressive, and the results we've obtained have been both reliable and reproducible. We used it in our ELISA assays with excellent results.
Advanced our research on thyroid hormone regulationExcellentIts high affinity for thyroxine has enabled us to achieve precise measurements in our experiments. The aptamer's performance has consistently exceeded our expectations, though we found that storing it at the recommended temperature is crucial to maintain its stability.
It has proven invaluableExcellentThe specificity and low background noise have streamlined our assays. However, it took some optimization to fit it perfectly into our existing protocol, but once that was done, it performed flawlessly.
We use the aptamer for a range of experimentsExcellentThe aptamer's performance has been consistent, and it has provided valuable insights into our research. My only suggestion would be to include more detailed protocols for its use in different types of experiments.
Overall, it's a valuable addition to our toolkitExcellentThe detection limits are excellent, and it has significantly improved our assay's sensitivity. However, we experienced some challenges with signal intensity in our assays, which required additional troubleshooting.