C1q Function Analysis Protocol

Creative Biolabs is one of the recognized specialists in the field of complement research. Our scientists pride themselves on their extensive experience to customize the best-fit strategy to meet your unique project requirements. Our highly skilled scientists are ready to start your project. Here, we briefly summarize the C1q function analysis protocols for our customers.

Overview of C1q

Complement protein C1q is considered to be the recognition molecule of the classical pathway, which can bind various ligands and play a crucial role in regulating the function of immune and non-immune cells. Various detection methods are closely linked with C1q functional analysis.

C1q Functional Assays

1. C1q hemolytic assays

In general, hemolytic assays are an important strategy for assessing the functional activity of the whole C1q molecule.

Fig.1 Flow chart of the C1q hemolytic assay protocol. (Creative Biolabs)

1. C1q-based inhibition of hemolysis assay

The functional activity of recombinant globular fragments of individual C1q chains is generally closely related to their ability to inhibit C1q-dependent hemolysis.

Fig.2 C1q-based inhibition of hemolysis protocol. (Creative Biolabs)

1. Enzyme-linked immunosorbent assay (ELISA) for C1q function analysis

Furthermore, the ligand-binding activity of C1q is usually tested by ELISA.

Fig.3 Interaction of C1q with human aggregated IgG detection protocol. (Creative Biolabs)

Based on an advanced technology platform, Creative Biolabs is committed to providing high-quality products and services to meet the needs of your complement research projects. With the increasing recognition of our products and related services by customers, Creative Biolabs has become a well-known biological brand in the world. With our continuous focus on the quality of complement-related products and services, we are proud of satisfying the needs of customers in more than 50 countries and regions both at home and abroad.

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1. Complement Products
2. Complement Test Services

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• Chromatography-Based C1q Purification Protocol
• Collagen-Like Region (CLR) of C1q Isolation Protocol
• gC1q Fragment Isolation Protocol
• C1q Fragments Expression and Purification Protocol
• C1q Biochemical Analysis Protocol
• C1q Hemolytic Assay Protocol
• C1q-Based Inhibition of Hemolysis Protocol
• Interaction of C1q with Human Aggregated IgG Detection Protocol