This product is an unconjugated anti-Human CD11c Monoclonal antibody (CTJS-819) generated from the Mouse. The antibody can be used for WB, IP, IF, ELISA.
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| Clonality | Monoclonal |
| Clone | CTJS-819 |
| Host Animal | Mouse |
| Isotype | IgG1 κ |
| Immunogen | The N-terminus of the human Integrin αX, a.a. 20-47 |
| Species Reactivity | Human; Mouse; Rat |
| Applications | WB; IP; IF; ELISA |
| Application Notes | WB: 1:100-1:10000 IP: 1-2 µg/mL IF: 1:50-1:500 ELISA: 1:30-1:3000 The optimal working dilutions should be determined by the end user. |
| Specificity | This antibody reacts with Human, Mouse, and Rat CD11c. |
| Purity | ≥95% as determined by SDS-PAGE |
| Conjugation | Unconjugated |
| Format | Liquid |
| Size | 200 µg |
| Storage | Store at 4°C. Do Not Freeze. |
| Type | Primary Antibody |
| Target Name | CD11c |
| Alternative Names | Integrin Subunit Alpha X; Integrin, Alpha X (Complement Component 3 Receptor 4 Subunit); Complement Component 3 Receptor 4 Subunit; Integrin Alpha-X; CD11C; Integrin Alpha X; CD11c Antigen; ITGAX; CD11c |
| Gene ID | 3687 |
| UniProt ID | P20702 |
| Introduction | Integrin alpha-X/beta-2 is a receptor for fibrinogen. It recognizes the sequence G-P-R in fibrinogen. It mediates cell-cell interaction during inflammatory responses. It is especially important in monocyte adhesion and chemotaxis. |
The specificity is top-notchExcellentIn our IP experiments, this CD11c monoclonal antibody has proven to be highly effective. The pull-downs are clean, and the antibody efficiently binds to its target. The lot-to-lot consistency is also commendable, ensuring reliable results across multiple experiments.
For immunofluorescence applications, this antibody is excellentExcellentIt stains CD11c-positive cells brightly without significant background staining. The images are crisp, allowing for clear visualization of cell surface markers. I've used it for several cell lines, and it has worked perfectly every time.
A dependable choice for our research needsExcellentWe use this antibody in our ELISA assays to detect CD11c expression, and it performs very well. The binding affinity is high, leading to sensitive and accurate quantification. The results are reproducible.
One of the best antibodies I've usedExcellentI have used this antibody for both WB and IF, and it has not disappointed. The bands in WB are sharp and specific, and the IF staining is bright and clear. It's an essential tool in my research on immune cell markers. The antibody is easy to work with and produces consistent results.
It's a high-quality reagent that I highly recommendExcellentI recently incorporated this antibody into my ELISA protocol, and the results have been outstanding. It has a high binding efficiency, which translates to more sensitive assays. The consistency across different batches is also impressive, ensuring reliable data over time.