Rat Anti-Human CD11b Monoclonal Antibody (CTJS-75)(Cat#: CTA-199)
This product is an unconjugated anti-Human CD11b Monoclonal antibody (CTJS-75) generated from the Rat. The antibody can be used for FC; IF; IHC; IP.
Summary
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Specifications
| Clonality |
Monoclonal |
| Clone |
CTJS-75 |
| Host Animal |
Rat |
| Isotype |
IgG2b |
| Immunogen |
T cell enriched splenocytes from B10 mice |
| Species Reactivity |
Human; Mouse; Rabbit |
| Applications |
FC; IF; IHC; IP |
| Application Notes |
FC: 1:50
IF: 1:10-1:500
IHC: 1:10-1:500
IP: 1:10-1:500
The optimal working dilutions should be determined by the end user. |
| Specificity |
This antibody reacts with Human, Mouse, Rabbit CD11b. |
| Purity |
≥95% as determined by SDS-PAGE |
| Format |
Liquid |
| Size |
25; 100 µg |
| Storage |
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. |
| Type |
Primary Antibody |
Target
| Target Name |
CD11b |
| Alternative Names |
Integrin Subunit Alpha M; Complement Component 3 Receptor 3 Subunit; Integrin Alpha-M; CR-3 Alpha Chain; CD11B ; CR3A; Complement Component Receptor 3, Alpha; Neutrophil Adherence Receptor; Antigen; CD11b (P170); CD11b Antigen; ITGAM; CD11b |
| Gene ID |
3684 |
| UniProt ID |
P11215 |
Information
| Introduction |
Integrin alpha M (ITGAM) is one protein subunit that forms the heterodimeric integrin alpha-M beta-2 (αMβ2) molecule, also known as macrophage-1 antigen (Mac-1) or complement receptor 3 (CR3). ITGAM is also known as CR3A, and cluster of differentiation molecule 11B (CD11B). The second chain of αMβ2 is the common integrin β2 subunit known as CD18, and integrin αMβ2 thus belongs to the β2 subfamily (or leukocyte) integrins.αMβ2 is expressed on the surface of many leukocytes involved in the innate immune system, including monocytes, granulocytes, macrophages, and natural killer cells. It mediates inflammation by regulating leukocyte adhesion and migration and has been implicated in several immune processes such as phagocytosis, cell-mediated cytotoxicity, chemotaxis and cellular activation. It is involved in the complement system due to its capacity to bind inactivated complement component 3b (iC3b). The ITGAM (alpha) subunit of integrin αMβ2 is directly involved in causing the adhesion and spreading of cells but cannot mediate cellular migration without the presence of the β2 (CD18) subunit.
In genomewide association studies, single nucleotide polymorphisms in ITGAM had the strongest association with systemic lupus erythematosus, with an odds ratio of 1.65 for the T allele of rs9888739 and lupus.
In histopathology, immunohistochemistry with antibodies against CD11B is frequently used to identify macrophages and microglia. |
For Research Use Only. Not for Diagnostic or Therapeutic Applications.
