Recombinant Human Clusterin Protein-6His tag(Cat#: CTP-123)
This product is a recombinant human Clusterin protein (26.7 kDa alpha chain and 24.2 kDa beta chain) with a C-terminal 6-His tag expressed in the mouse myeloma cell line, NS0. It is available for Bioactivity Assays.
Summary
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Specifications
| Expressed Host |
Mouse myeloma cell line, NS0 |
| Specie Reactivity |
Human |
| Target |
Clusterin |
| Molecule Mass |
26.7 kDa ( alpha chain) and 24.2 kDa ( beta chain) |
| Purity |
>90% by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining |
| Endotoxin |
<1.0EU per 1µg (determined by the LAL method) |
| Formulation |
PBS |
| Applications |
Bioactivity Assays |
| Product Form |
Lyophilized |
| Storage |
Store at 2 to 8°C after reconstitution for short term (1-2 weeks). For long term storage, aliquot and store at -20 or -80°C. Avoid repeated freezing and thawing cycles. |
Protein Information
| Introduction |
Clusterin isoform 1 functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress-induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. Secreted isoform 1 protects cells against apoptosis and against cytolysis by complement. Intracellular isoforms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Nuclear isoforms promote apoptosis. Mitochondrial isoforms suppress BAX-dependent release of cytochrome c into the cytoplasm and inhibit apoptosis. Plays a role in the regulation of cell proliferation. |
| Alternative Names |
Testosterone-Repressed Prostate Message; Clusterin (Complement Lysis Inhibitor, SP-40, 40, Sulfated Glycoprotein 2, Testosterone-Repressed Prostate Message 2, Apolipoprotein J); Apolipoprotein J; Complement-Associated Protein SP-40, 40; Complement Cytolysis Inhibitor; Complement Lysis Inhibitor; Sulfated Glycoprotein 2; Ku70-Binding Protein 1; NA1/NA2; TRPM-2; APOJ; KUB1; CLI; Aging-Associated Gene 4 Protein; Aging-Associated Protein 4; APO-J; SGP-2; SP-40; TRPM2; Apo-J; AAG4; CLU1; CLU2; SGP2 |
| Gene ID |
1191 |
| UniProt ID |
P10909 |
For Research Use Only. Not for Diagnostic or Therapeutic Applications.
