This product is a recombinant rat C4A protein with a N-terminal 10XHis tag and C-terminal Myc tag tag expressed in the E.coli/Mammalian cell/Baculovirus. It is availible for WB, ELISA.
Expressed Host | E.coli/Mammalian cell/Baculovirus |
Specie Reactivity | Rat |
Target | C4A |
Purity | >85 % determined by SDS-PAGE |
Endotoxin | <1.0EU per 1µg (determined by the LAL method) |
Formulation | Tris-based buffer,50% glycerol |
Applications | WB; ELISA |
Product Form | Liquid |
Storage | Store at -20°C/-80°C. Avoid freeze-thaw cycles. |
Introduction | Non-enzymatic component of C3 and C5 convertases and thus essential for the propagation of the classical complement pathway. Covalently binds to immunoglobulins and immune complexes and enhances the solubilization of immune aggregates and the clearance of IC through CR1 on erythrocytes. C4A isotype is responsible for effective binding to form amide bonds with immune aggregates or protein antigens, while C4B isotype catalyzes the transacylation of the thioester carbonyl group to form ester bonds with carbohydrate antigens. Derived from proteolytic degradation of complement C4, C4a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. |
Alternative Names | Complement C4A (Rodgers Blood Group); C3 And PZP-Like Alpha-2-Macroglobulin Domain-Containing Protein; Complement Component 4A (Rodgers Blood Group); Acidic Complement C4; Complement C4-A; C4S; MHC Class III Region Complement; Complement Component 4A; Rodgers Form Of C4; C4A Anaphylatoxin; C4AD; C4A |
Gene ID | 24233 |
UniProt ID | P08649 |