Collectin-10

Background

Collectin-10 (CL-10), also known as collectin liver 1 (CL-L1) or Collectin-34 (CL-34), is a protein encoded by the gene COLEC10. It belongs to the family of collagenous Ca²⁺-dependent (C-type) lectins which contains triple-helical collagen-like domains as well as C-terminal Ca²⁺-dependent lectin domains. In addition to CL-10, other eight collectins have been identified, including mannose-binding lectin (MBL), surfactant proteins A and D (SP-A and SP-D), collectin placenta 1 (CL-12), conglutinin, collectins of 43kDA and 46 kDa, and collectin-11. These proteins are highly conserved in evolution, with the same structural characteristics as collagen and the same functional properties as lectins.

CL-10 is one of the most recently discovered collectins. It is primary expressed in the liver and placenta. It involves a series of steps of the lectin complement pathway which plays an important role in the migration of cells to form organs and systems of the body during development before birth. Besides, CL-10 appears to be particularly important in the regulation of immune responses. CL-11 and CL-10 have been shown to form hetero-oligomers which circulate in the serum and binds to mannose-binding lectin (MBL)-associated serine proteases (MASPs), involved in the complement activation in a MASP-2-dependent fashion. CL-10 has also revealed to bind to carbohydrate antigens on microorganisms such as mannose, fucose, and galactose with high affinity, facilitating the complement activation against foreigners.

Collectin-10 Functional Service

Creative Biolabs offers a diverse selection of collectin-10-specific products, including anti-collectin-10 antibodies, ELISA kits, and complement collectin-10 proteins. These carefully crafted resources are crucial in advancing research projects that aim to develop therapeutic strategies for a wide range of diseases.

Fig.1 Expression levels of C-type lectins Collectin-10 and Collectin-11 in inactive hepatic stellate cells.¹

Researchers have demonstrated that hepatic stellate cells, major nonparenchymal cells in the liver, activate in response to liver injury, contributing to fibrosis through extracellular matrix production. Single-cell RNA sequencing, with its high-resolution capabilities, has unveiled subpopulations and functions of these cells. Re-analysis of sequencing data and pseudotime trajectory inference revealed that C-type lectins, such as Collectin-10 and Collectin-11, are chiefly expressed by quiescent hepatic stellate cells in mouse livers, not hepatocytes. Collectin-10 expression diminishes in fibrotic livers. In human livers, collectin-10 follows a similar expression pattern, decreasing as fibrosis progresses. collectin-10 influences inflammation, angiogenesis, and matrix alterations, paradoxically promoting extracellular matrix gene expression in vitro. Elevated serum collectin-10 levels in chronic liver disease patients correlate with D-dimer concentrations, underscoring collectin-10’s role in liver fibrosis pathogenesis.

Creative Biolabs offers a diverse range of services centered on collectin-10, including binding assessments and additional functional analyses, carefully tailored to support our esteemed clients in both clinical and research endeavors.

References

1. Zhang, Mengfan, et al. "The C-type lectin COLEC10 is predominantly produced by hepatic stellate cells and involved in the pathogenesis of liver fibrosis." Cell Death & Disease 14.11 (2023): 785. Distributed under Open Access license CC BY 4.0, without modification.