VBS++ (with Ca and Mg) Buffer(Cat#: CTR-004)
VBS++ is like the traditional buffer used in complement assays (GVB++) but it lacks the carrier protein gelatin often used in these assays. GVB may be replaced with VBS if gelatin is undesirable or may interfere with the assays. The concentrations of metal ions are the traditional concentrations and these give maximal complement activation.
Summary
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Specifications
| Application |
VBS++ may be used when a buffer free of carrier proteins is needed for diluting proteins or as the buffer for complement assays. VBS++ may also be used to prepare GVBo, GVB++, and GVBE or it may be used to prepare similar complement buffers that include different carrier proteins in place of gelatin in situations where gelatin interferes with assays. BSA (0.1%) or another carrier protein may be used for this. |
| Composition |
5 mM Veronal, 145 mM NaCl, 0.025 % NaN3, pH 7.3. Containing 0.15 mM calcium chloride and 0.5 mM magnesium chloride. |
| Form |
Liquid |
| Storage |
Store at 4°C and avoid freezing. |
For Research Use Only. Not for Diagnostic or Therapeutic Applications.
