Based on the optimized and validated surface display platform, Creative Biolabs provides clients high-class CreMap™ epitope mapping services to help avoid the pitfalls of variability in assays and benefit from the efficiency and accuracy of a dedicated facility.
Detailed information on epitopes is of great importance both for the understanding of immunological events and for the development of effective vaccine and diagnostic tools against a vast number of diseases. Among all the methods available, the surface display technology provides a powerful solution for epitope mapping due to their relative cheapness and quickness.
The key principle of display techniques is testing the binding ability of a variety of peptides presented on the display platforms to the antibody of interest. The antigen DNA is fragmented by enzymes, expressed on the surface of the display platform to generate a library. After that, the binding ability of the peptide displayed on the phage surface to the antibody would be tested and the epitope mapping would be performed. One of the exclusive features of surface display technique is that the peptide which is expressed by the fragment containing the epitope would be recognized by the antibody. Thus, the information of the DNA fragment will be obtained simultaneously by sequencing back to the DNA fragment.
Phage display is one of the most frequent and popular display methods. Construction of phage display peptide libraries represents a popular way for screening epitopes. This powerful approach involves fusion of the foreign DNA fragments with the filamentous phage gene coding coat protein (e.g. pIII, pVI, pVII, pVIII, and pIX). In the case of M13 filamentous phage display, the peptides of interest are more often ligated into pIII gene. There are around five copies of pIII gene in M13 filamentous phage. This format works well and the general selection may not fall short when we are seeking high-affinity binders. The incorporation of peptides does not inhibit the phage cycle.
Fig.1 Schematic of the filamentous phage for recombinant display of foreign peptide.
Fig.2 Workflow for surface display technique.
With extensive experience in epitope mapping service, Creative Biolabs is dedicated to helping clients determining epitopes with well-established surface display platform. If you are now doing research on antigen-antibody complex, trying to figure out epitopes on the antigen, or if you are interested in our surface display platform, please contact usand a formal feedback will be sent back as soon as possible.