Creative Biolabs is offering the most comprehensive services for antibody development projects. With strict regulation and effective execution, we are dedicated to providing the most valuable solutions to complete your projects.
HighlightsWith over a decade of experience in phage display technology, Creative Biolabs can provide a series of antibody or peptide libraries that are available for licensing or direct screening. These ready-to-use libraries are invaluable resources for isolating target-specific binders for various research, diagnostic or therapeutic applications. Highlights
Creative Biolabs has established a broad range of platforms for developing novel antibodies or equivalents. These cutting-edge technologies enable our scientists to meet your demands from different aspects and tailor the most appropriate solution that contributes to the success of your projects.
HighlightsWith deep understanding in antibody-related realms and extensive project experience, Creative Biolabs offers a variety of references to help you learn more about our capacities and achievements, including infographic, flyer, case study, peer-reviewed publications, and all kinds of knowledge that can assist your projects. You are also welcome to contact us directly for more specific solutions.
HighlightsGet a real taste of Creative Biolabs, one of the most professional custom service providers in the world. We are committed to providing highly customized comprehensive solutions with the best quality to advance your projects.
HighlightsBased on the optimized and validated surface display platform, Creative Biolabs provides clients high-class CreMap™ epitope mapping services to help avoid the pitfalls of variability in assays and benefit from the efficiency and accuracy of a dedicated facility.
Detailed information on epitopes is of great importance both for the understanding of immunological events and for the development of effective vaccine and diagnostic tools against a vast number of diseases. Among all the methods available, the surface display technology provides a powerful solution for epitope mapping due to their relative cheapness and quickness.
The key principle of display techniques is testing the binding ability of a variety of peptides presented on the display platforms to the antibody of interest. The antigen DNA is fragmented by enzymes, expressed on the surface of the display platform to generate a library. After that, the binding ability of the peptide displayed on the phage surface to the antibody would be tested and the epitope mapping would be performed. One of the exclusive features of surface display technique is that the peptide which is expressed by the fragment containing the epitope would be recognized by the antibody. Thus, the information of the DNA fragment will be obtained simultaneously by sequencing back to the DNA fragment.
Phage display is one of the most frequent and popular display methods. Construction of phage display peptide libraries represents a popular way for screening epitopes. This powerful approach involves fusion of the foreign DNA fragments with the filamentous phage gene coding coat protein (e.g. pIII, pVI, pVII, pVIII, and pIX). In the case of M13 filamentous phage display, the peptides of interest are more often ligated into pIII gene. There are around five copies of pIII gene in M13 filamentous phage. This format works well and the general selection may not fall short when we are seeking high-affinity binders. The incorporation of peptides does not inhibit the phage cycle.
Fig.1 Schematic of the filamentous phage for recombinant display of foreign peptide.
Fig.2 Workflow for surface display technique.
Key Features
With extensive experience in epitope mapping service, Creative Biolabs is dedicated to helping clients determining epitopes with well-established surface display platform. If you are now doing research on antigen-antibody complex, trying to figure out epitopes on the antigen, or if you are interested in our surface display platform, please contact usand a formal feedback will be sent back as soon as possible.
All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.
