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CreMap™ Epitope Mapping by Surface Plasmon Resonance

Creative Biolabs helps to figure out epitopes by providing mapping services with Surface Plasmon Resonance (SPR) technology that is quite useful in the evaluation of antigen-antibody interaction, affinity measurement, competition assays, ligand fishing, ADME (adsorption, distribution, metabolism, and excretion) characterization.

SPR is the resonant oscillation of conduction electrons at the interface between negative and positive permittivity material stimulated by incident light. SPR allows for the analysis of the association and dissociation rate constants and modeling of biomolecular interaction kinetics. For epitope mapping analysis, the antigen is typically immobilized on a sensor chip surface, and the antibody flows through a microfluidic system in contact with the chip surface. SPR measures in real time the changes in mass bound to the sensor chip. It detects the changes in refractive index of the surface layer of a solution in contact with the sensor chip that is caused by a variation of the mass on the sensor chip surface. SPR immunoassay resembles the concept of the ELISA technique, but it is label-free, eliminating the requirement for reporter molecules or tags which could be time-consuming and even alter the molecular interaction.

CreMap™ Epitope Mapping by Surface Plasmon Resonance
Fig.1 The schematic of the surface plasmon resonance measurement.

Method

Surface plasmon resonance (SPR) is a label-free optical sensing technique to characterize binding between a soluble ligand and a surface-immobilized receptor.


Key Features


SPR is a powerful method for characterizing the epitopes on the antigen.Scientists from Creative Biolabs have been working on developing and optimizing our SPR platform to better serve growing demands of customers around the world. If you are trying to figure out epitopes on the antigen, or if you are interested in our platform, please feel free tocontact us by sending E-mail. A formal feedback will be sent back as soon as possible.

References

  1. Wittenberg, N.J., et al., Applications of SPR for the characterization of molecules important in the pathogenesis and treatment of neurodegenerative diseases. Expert Rev Neurother, 2014. 14(4): p. 449-63.
  2. Cooper, M.A., Optical biosensors in drug discovery. Nat Rev Drug Discov, 2002. 1(7): p. 515-28.
  3. Stahelin, R.V., Surface plasmon resonance: a useful technique for cell biologists to characterize biomolecular interactions. Mol Biol Cell, 2013. 24(7): p. 883-6.
  4. Myszka, D.G., Kinetic analysis of macromolecular interactions using surface plasmon resonance biosensors. Curr Opin Biotechnol, 1997. 8(1): p. 50-7.



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