Creative Biolabs is offering the most comprehensive services for antibody development projects. With strict regulation and effective execution, we are dedicated to providing the most valuable solutions to complete your projects.
HighlightsCreative Biolabs is offering the most comprehensive services for antibody development projects. With strict regulation and effective execution, we are dedicated to providing the most valuable solutions to complete your projects.
HighlightsCreative Biolabs has established a broad range of platforms for developing novel antibodies or equivalents. These cutting-edge technologies enable our scientists to meet your demands from different aspects and tailor the most appropriate solution that contributes to the success of your projects.
HighlightsWith deep understanding in antibody-related realms and extensive project experience, Creative Biolabs offers a variety of references to help you learn more about our capacities and achievements, including infographic, flyer, case study, peer-reviewed publications, and all kinds of knowledge that can assist your projects. You are also welcome to contact us directly for more specific solutions.
HighlightsGet a real taste of Creative Biolabs, one of the most professional custom service providers in the world. We are committed to providing highly customized comprehensive solutions with the best quality to advance your projects.
Highlights| Protein Name | Corticotropin releasing hormone receptor 2 |
| Gene Name | CRHR2 |
| Uniprot | Q13324 (Human); Q60748 (Mouse) |
| Synonym | CRF2; CRFR2; CRF-RB; HM-CRF; corticotropin-releasing factor receptor 2; CRH receptor 2 variant B; CRH-R2 |
| Background | CRHR2, encoded by CRHR2 gene, also known as CRF2, is a cell-surface protein. It belongs to G-protein coupled receptor family which has seven transmembrane domains. The extracellular domain of receptor responds to peptide hormones, corticotropin-releasing hormone (CRH) and urocortin (UCN), involved in the regulation of response to stress. Most studies have been designed to investigate the CRHR2 possible role in regulating social behavior response to stress. |
Creative Biolabs offers comprehensive range of membrane protein products empowers your unique research needs.
| CAT# | Product Name | Expression System | Protein Length | Solubilizing Agents |
| S01YF-1023-KX286 | NativeExtract™ Human CRHR2 Membrane Protein (Full length, Super Nanodisc) | HEK293 cells | Full length | Native Nanodisc |
| MPX3525K | MemDX™ Membrane Protein Human CRHR2 Expressed in vitro E.coli expression system, Full Length of Mature Protein | E.coli cell-free | Full length | Detergent |
| MP0268X | MemDX™ Membrane Protein Human CRHR2 (Corticotropin releasing hormone receptor 2) for Antibody Discovery | Wheat germ cell-free | Full length | N/A |
| Please for more products or customization | ||||
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05/Aug/2023
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16/Jun/2024
We provide tailored solutions to meet your specific requirements. Please for more services.
Quantitative RT-PCR
Creative Biolabs offers quantitative RT-PCR services. The precise quantification of specific gene transcripts frequently employs quantitative reverse transcriptase polymerase chain reaction (qRT-PCR), a ubiquitous methodology in molecular biology. In investigations specifically addressing the CRHR2, the requisite human tissue complementary DNA (cDNA) is typically procured from commercial repositories. Subsequently, total RNA is meticulously isolated from these tissue samples, often facilitated by standardized extraction kits. Following this crucial isolation step, the extracted RNA undergoes enzymatic conversion into cDNA via reverse transcription is commonly employed for this purpose. For accurate comparative analysis, the resultant gene expression data are invariably normalized against a suitable endogenous control gene.
Creative Biolabs offers comprehensive and innovative services to drive the development of CRHR2 cell therapy. Please for more services.
HEK293 CRHR2 and FAM Microscopy Studies
Creative Biolabs offers HEK293-CRHR2 and FAM microscopy studies services. Microscopic investigations are indispensable for discerning the subcellular localization and intracellular trafficking pathways of the CRHR2 within cellular contexts. Typically, experimental paradigms involve the transient transfection of HEK293 cells with expression vectors encoding specific CRHR2 constructs. Subsequently, these cells are subjected to treatment with defined agonists, thereby initiating receptor activation and subsequent cellular responses. Following the stimulation period, cells undergo chemical fixation, a crucial step for preserving cellular architecture, and are then immunolabeled with highly specific primary and fluorophore-conjugated secondary antibodies targeting the protein of interest. The resulting fluorescent signals are meticulously captured and analyzed using confocal laser scanning microscopy. This rigorous methodological approach, therefore, yields critical insights into the dynamic cellular behavior and distribution of CRHR2.
Phosphoproteome Quantification
Creative Biolabs offers phosphoproteome quantification services. The comprehensive identification and precise quantification of phosphorylated proteins integral to CRHR2 signal transduction cascades are routinely achieved through phosphoproteomic analysis. This advanced methodological approach typically leverages Tandem Mass Tag (TMT) isobaric labeling strategies in conjunction with high-resolution mass spectrometry. Such a combination enables the accurate determination of phosphorylation stoichiometries across a wide array of proteins. Subsequent to data acquisition, the raw mass spectrometry data undergo rigorous computational processing. Ultimately, robust statistical methodologies are applied to discern significant alterations in protein phosphorylation profiles that occur in response to, or are perhaps directly mediated by, CRHR2 activation.
Zebrafish Mutant Models
Creative Biolabs offers zebrafish mutant models development services. The developmental and functional contributions of the CRHR2 are frequently investigated using Danio rerio, or zebrafish, as a salient vertebrate model system. To this end, specific CRHR2 mutant zebrafish lines are meticulously generated, thereby enabling the assessment of its physiological significance. Subsequent phenotypic analyses are then rigorously conducted on these genetically modified organisms. Such examinations are designed to systematically evaluate the pleiotropic effects stemming from a deficiency in CRHR2, encompassing observable alterations in complex behavioral paradigms, shifts in downstream gene expression profiles, and perhaps even modulated neuroinflammatory responses.
All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.
