Introduction of CXCR5
CXCR5 is a G protein-coupled receptor in the CXC chemokine receptor family, encoded by CXCR5 gene. It is one of seven CXC chemokine receptors (CXCR1-CXCR7) in mammals. The receptors can recognize CXC chemokine that possesses an E-L-R amino acid motif. CXCR5 is specifically expressed in mature B-cell and Burkitt's lymphoma. Recently, most studies focus on the role of CXCR5 in the pathological process of cancer.
|Basic Information of CXCR5|
|Protein Name||C-X-C chemokine receptor type 5|
|Aliases||BLR1, CD185, MDR15|
|Organism||Homo sapiens (Human)|
Function of CXCR5 Membrane Protein
CXCR5 can bind to B-lymphocyte chemoattractant (BLC), also known as CXCL13, and is involved in B-cell migration into B-cell follicles of spleen and Peyer patches. CXCL13/CXCR5 plays an essential role in the progression of multiple diseases. In neurosyphilis patients, CXCL13/CXCR5 mediates the aggregation of B cells, resulting in ectopic germinal centers (EGCs) formation in the intracranial syphilitic gumma, inducing the abnormal humoral immune response. Besides, the expression of CXCR5 is associated with the pathogenesis of multiple tumors, such as colorectal cancer, breast cancer, prostate cancer, and chronic lymphocytic leukemia. The elevated CXCR5 expression may be observed in breast tumors that may contribute to abnormal cell survival and migration. Similarly, the expression of CXCR5 can promote the pathogenesis, development, metastasis, and relapse of colorectal cancer. It also suggests that blockade of CXCR5 may serve as an effective therapy for tumors. Recently, more and more studies focus on the role of CXCR5 in the pathological process of cancer, expecting to obtain a new therapeutic strategy for cancer.
Fig. 1 Reciprocal cross-talk between CLL cells and FDCs in the germinal center. (Lopezguerra, 2014)
Application of CXCR5 Membrane Protein in Literature
The research suggests that CXCL13 inhibitor reduce the volume and growth of tumor as well as the mRNA and protein expression levels of key members (CXCL13, CXCR5, and ERK) of the CXCR5/ERK signaling pathway. So authors propose the recession of tumors induced by CXCL13 inhibitor may be associated with the CXCR5/ERK signaling pathway.
The study shows that the overexpression of CXCR5 increases the ability of mesenchymal stromal cells (MSCs) to respond to migratory stimuli and highly intensifies their immunomodulatory effects in vivo. This strategy for enhancing targeted stem/progenitor cell homing may improve the efficacy of MSC-based therapies.
The research aims to investigate the role of miR-192 in the differentiation of T follicular helper cells in childhood asthma. Results show that miR-192 blocks the activation pathway of Tfh cells by targeting CXCR5, which is a reasonable cellular target for therapeutic intervention.
The study shows that a susceptible allele for Sjögren's syndrome (SS) is linked with decreased CXCR5 expression. And the decrease of CXCR5+ cells in circulation is also related to homing of B and T cells to the autoimmune target organ. These suggest that CXCR5/CXCL13 axis may be a useful therapeutic drugs target for SS.
The study identifies that the effects of NFkB on the CXCR5 promoter inversely correlated with p63 and p73 levels via deletion analysis and site-directed mutagenesis. Thus all three p53 family members are involved in the mediation of the effects of genotoxic stress on the CXCR5 promoter using the same mechanism associated with attenuation of NFkB activity.
CXCR5 Preparation Options
To obtain the soluble and functional target protein, the versatile Magic™ membrane protein production platform in Creative Biolabs enables many flexible options, from which you can always find a better match for your particular project. Aided by our versatile Magic™ anti-membrane protein antibody discovery platform, we also provide customized anti-CXCR5 antibody development services.
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