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DARPin Scaffold Library Construction Service

Creative Biolabs provides custom scaffold library construction services for DARPin, a non-immunological alternative for traditional Ig-based scaffolds. Our innovative scientists have developed a powerful HiAffi™ phage display platform for the generation of scaffold libraries with satisfactory controllability and operability.

Designed ankyrin repeat proteins (DARPins) are a novel class of small nonimmunoglobulin protein scaffold derived from natural ankyrin repeat proteins. Ankyrin repeat proteins are one of the most common binding proteins in nature and responsible for diverse functions, such as cell signaling and receptor binding. The basic structure of ankyrin repeat proteins is formed by a β-turn followed by two antiparallel α-helices and a loop connected to the β-turn of the next repeat. DARPin is composed of a number of repeat motifs proteins, usually consists of four or five repeats, which is about 14 kDa or 18 kDa.

Crystal structure of DARPin (PDB 2JAB)
Fig.1 Crystal structure of DARPin (PDB 2JAB)

DARPins are featured by their large and modular target interaction surfaces. A unique feature of such surfaces is that they can be adapted to the target size by adjusting the number of repeats in the protein. Successful examples with high affinity against a variety of targets have been reported. It is suggested that DARPins are suited not only for novel fusions and conjugates and extracellular targeting use but also for intracellular applications, because of their high thermodynamic stability, reversible folding behavior, high solubility, high yield in E. coli, the absence of cysteines and low aggregation tendencies. Therefore, DARPins is considered to offer advantages over antibodies in the discovery and development of drug therapy.

So far, various DARPins have been selected through display technology and the use of the SRP (signal recognition particle) pathway also ensures the efficient display of stable and fast-folding DARPins on phage particles. During the construction of DARPin library, naturally conserved residues would be defined as invariant framework residues and variable amino acid positions can be subjected to randomization. The randomized positions were fixed according to sequence statistics and structural considerations. In this case, the ankyrin repeat positions 2, 3, 5, 13, and 14 were subjected to randomization, randomized position 33 of the library was rarely involved in protein interactions and thus defined as invariant serine.

Based on our proprietary Hi-Affi™ phage display platform, Creative Biolabs is able to rapidly generate DARPin libraries with desired diversity or multi-DARPins. The diversity of formats and robustness of these multi-DARPins have great potential to overcome a limitation of current therapeutic approaches for a chosen disease. This will again significantly speed up the R&D process in drug invention. DARPins library construction service of Creative Biolabs with randomized potential target interaction residues resulting in a diversity of over 1010 variants have been generated through our phage display technology, which satisfies most research needs. What’ more, our DARPin libraries have very favorable biophysical properties. They are very well expressed, monomeric in solution, highly soluble, thermodynamically stable and shows fast cooperative folding behavior which indicate a wide range of applications for the biological research.

DARPin Scaffold Library Construction Service

Features of our Hi-Affi™ platform:

Creative Biolabs commits to the research and development of scaffold protein area. Combining with our well-recognized Hi-Affi™ phage display platform, our scientists are confident in offering 100% precise mutant library construction with the expected size of over 1010 to meet our global clients’ most specific research objective. If you are interested in our DARPin scaffold library construction service, please do not hesitate to inquire us for more details.

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