Creative Biolabs is offering the most comprehensive services for antibody development projects. With strict regulation and effective execution, we are dedicated to providing the most valuable solutions to complete your projects.
HighlightsWith over a decade of experience in phage display technology, Creative Biolabs can provide a series of antibody or peptide libraries that are available for licensing or direct screening. These ready-to-use libraries are invaluable resources for isolating target-specific binders for various research, diagnostic or therapeutic applications. Highlights
Creative Biolabs has established a broad range of platforms for developing novel antibodies or equivalents. These cutting-edge technologies enable our scientists to meet your demands from different aspects and tailor the most appropriate solution that contributes to the success of your projects.
HighlightsWith deep understanding in antibody-related realms and extensive project experience, Creative Biolabs offers a variety of references to help you learn more about our capacities and achievements, including infographic, flyer, case study, peer-reviewed publications, and all kinds of knowledge that can assist your projects. You are also welcome to contact us directly for more specific solutions.
HighlightsGet a real taste of Creative Biolabs, one of the most professional custom service providers in the world. We are committed to providing highly customized comprehensive solutions with the best quality to advance your projects.
HighlightsCreative Biolabs has established an advanced surface plasmon resonance (SPR) platform to determine the interaction between therapeutic antibodies (like IgGs) and fragment crystallizable γ receptors (FcγR) or neonatal Fc receptor (FcRn). Through our high-throughput system, clients could accelerate their research with the most comprehensive view of therapeutic antibodies antibody and with reducing project costs and timelines.
The Interaction Between Therapeutic Antibodies and FcγRs/FcRn
Different FcγR subclasses are known to be present on human effector cells: the high-affinity FcγRI (CD64) and the low-affinity receptors FcγRIIa (CD32a), FcγRIIb (CD32b), FcγRIIIa (CD16a), and FcγRIIIb (CD16b). Within these five subclasses, different polymorphic variants exist, and in some cases, they can influence binding of IgG to these receptors. Therapeutic antibodies are one of the largest classes of modern biopharmaceuticals. Interactions of IgGs with effector cells through FcγRs are often considered a mode of action of therapeutic antibodies. FcγRs are cell surface receptors that can be found on innate immune effector cells such as natural killer cells and macrophages. A therapeutic IgG binds to a membrane-bound antigen on target cells by its complementary-determining regions (CDRs) in the variable domain, while the Fc region in the constant domain can bind to various FcγRs on effector cells, which could lead to effector function, like antibody-dependent cellular cytotoxicity (ADCC) or phagocytosis (ADCP). The binding kinetics and affinities of IgG-FcγR interactions are hence important parameters for understanding FcγR-mediated immune function. Furthermore, FcRn determines the half-life of IgGs in the bloodstream. Binding of Fc receptor to IgG takes place in the endosome at acidic pH, and the IgG is then recycled back into plasma at neutral pH, thereby preventing lysosomal degradation. Therefore, binding of therapeutic antibodies to FcγRs/FcRn should be evaluated as part of the critical quality attribute (CQA) assessment.
Determination of IgG-FcγRn/FcRn Interaction by SPR in Creative Biolabs
SPR is an extremely sensitive method to detect molecular interactions by tracking the change of signal via sensor chips. Aiming at rapid measurements of IgG samples for high-throughput screening purposes, scientists from Creative Biolabs have developed several SPR methods for FcγRn or FcRn binding to determine IgG-FcγRn/FcRn interaction. General binding response includes three phases: association phase, equilibrium phase, and dissociation phase. Monitoring the changes in the SPR signal over time results in a plot of the binding response versus time called sensorgram. Fitting of the sensorgram data allows calculation of binding parameters, such as the association (Ka) and dissociation (Kd) rate constants and ultimately determine the binding affinity (KD).
Key Advantages
Creative Biolabs is pleased to share our cutting-edge technology and extensive expertise in the determination of IgG-FcγRn/FcRn interaction by SPR to facilitate our clients’ research and project development. We can offer high-quality customized SPR services by adjusting protocols to meet every unique requirement. Please feel free to contact us for more information.
All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.
