ABCG8 Analysis Service

At Creative Biolabs, we provide an integrated suite of biomarker assays, which are indispensable for validating targets, predicting therapeutic efficacy, and ensuring safety. Our comprehensive portfolio, along with our scientific and regulatory expertise, fully supports your program from start to finish. Find the ABCG8 analysis service that fits your needs.

Introduction of ABCG8

Located on chromosome 2p21, the ABCG8 gene has 13 exons that encode 673 amino acid residues. As a member of the ATP-binding cassette (ABC) transporter superfamily, ABCG8 plays a pivotal role in the regulation of intestinal plant sterol absorption by actively exporting plant sterols back into the intestinal lumen. This process is a critical frontline defense against the over-absorption of phytosterols.

Fig.1 Structure and SNP of ABCG5/G8.¹

The ABCG5/ABCG8 transporters are functional heterodimers that are co-expressed in both the canalicular membrane of the hepatocyte and the apical membrane of the enterocytes. They work in concert to export cholesterol and phytosterols, thereby excluding them from systemic circulation. The formation of this heterodimer is an absolute necessity to direct the complex from the endoplasmic reticulum to the apical membrane, ensuring proper function. Mutations in either of these half-transporters can lead to defective biliary sterol transport and result in the rare human disease known as sitosterolemia.

ABCG8 Analysis Services at Creative Biolabs

Creative Biolabs offers a range of sophisticated services to provide a complete overview of the ABCG8 pathway, from genetic variations to protein function and localization. We provide the highest quality and most specific data to guide your critical decisions in drug development and diagnostics. Our team has validated our in-house developed anti-ABCG8 antibodies using multiple techniques, including Western blotting, ELISA, IHC, ICC, and IF with known positive and negative samples to ensure specificity and high affinity.

Our popular services targeting ABCG8 include, but are not limited to, the following key areas:

• ABCG8 Genetic Variation Analysis

We identify pathogenic mutations and single nucleotide polymorphisms (SNPs) within the ABCG8 gene, providing insight into an individual's genetic predisposition and therapeutic response.

• ABCG8 Protein Identification & Localization

Our assays are designed to precisely identify and localize ABCG8 protein expression in specific tissues and cellular compartments, which is crucial for understanding its functional role.

• Interaction Evaluation

We assess the critical interaction between ABCG8 and ABCG5, as well as with other potential binding partners or compounds, to help elucidate complex signaling pathways.

• Functional Transport Assays

Our cell-based models provide in vitro quantification of ABCG8 transport activity, measuring sterol efflux and uptake to determine the efficacy of your compounds.

Service Workflow

Our streamlined service workflow is designed for efficiency and collaboration. The process is clear and transparent from start to finish.

Applications

Service Highlights

• Validated Antibodies: Our anti-ABCG8 antibodies have undergone rigorous validation, ensuring exceptional specificity and high affinity across multiple applications.
• Integrated Solutions: We offer a comprehensive suite of services, from genetic analysis to functional assays, providing a holistic view of the ABCG8 pathway.
• Technical Expertise: Our team has decades of experience in biomarker analysis and is dedicated to providing expert guidance and support throughout your project.
• Customizable Service: We tailor our analytical services to fit your specific research needs, ensuring that you receive the most relevant and actionable data.

FAQs

1. What are the most common challenges customers face when analyzing ABCG8, and how do you help overcome them?

   Customers frequently face issues with antibody specificity, low expression levels, and the complexity of analyzing a heterodimeric protein. We address these by using our validated, high-affinity antibodies, employing sensitive detection methods, and providing expert consultation to design a robust experimental strategy.

2. How do your functional transport assays for ABCG8 differ from those offered by other providers?

   Our functional assays utilize advanced cell-based models that closely mimic in vivo conditions, allowing for accurate quantification of sterol efflux and uptake. We provide a highly controlled environment and precise measurement tools to ensure your data is both accurate and biologically meaningful, offering a superior alternative to standard methods.

3. Can your services help us screen for drug candidates that modulate ABCG8 activity?

   We offer a high-throughput compound screening service to identify activators or inhibitors of the ABCG8 pathway. Our platform rapidly screens compound libraries and provides detailed dose-response data, essential for identifying promising candidates and optimizing therapeutic potential early in the discovery process.

4. How can your genetic analysis services for ABCG8 support clinical research and diagnostics?

   Our genetic services support clinical research by identifying SNPs and pathogenic mutations in the ABCG8 gene linked to sitosterolemia and other metabolic disorders. This information can be used to develop diagnostic tests for patient stratification and to predict an individual's response to therapy.

5. Is it possible to evaluate the co-localization and interaction of ABCG5 and ABCG8 using your services?

   Yes, our services are designed to evaluate the co-localization and critical interaction between ABCG5 and ABCG8. We utilize advanced techniques like co-immunoprecipitation and immunofluorescence, which provide visual and quantitative data on their co-expression and dimerization.

6. Can you analyze ABCG8 expression in rare or difficult-to-obtain tissue samples?

   Our highly sensitive and validated methods are well-suited for analyzing ABCG8 expression in rare or difficult-to-obtain tissue samples. We provide specialized protocols for sample handling, and our expertise ensures we extract the maximum high-quality data from your valuable specimens.

If you are interested in our analysis services or need a tailored solution, please feel free to contact us for more information.

Reference

1. Xavier, Bala M., et al. "Transmembrane polar relay drives the allosteric regulation for ABCG5/G8 sterol transporter." International journal of molecular sciences 21.22 (2020): 8747. Distributed under Open Access license CC BY 4.0, without modification. https://doi.org/10.3390/ijms21228747

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