ABLIM3 analysis is critical for identifying its role in cell proliferation, migration, and metastasis. Its expression patterns can serve as potential biomarkers for diagnosis, prognosis, and therapeutic response in various cancers.
At Creative Biolabs, our expert team provides an advanced platform for singleplex and multiplex biomarker analysis. We offer a variety of tailored strategies to help clients overcome the challenges of traditional methods. Our ABLIM3 analysis service guarantees the highest discovery power for maximum research impact.
Introduction of ABLIM3
The ABLIM3 gene encodes a member of the actin-binding LIM family of proteins, characterized by a unique N-terminal LIM domain and a C-terminal domain. The protein encoded by this gene interacts with actin filaments and is a crucial component of adherens junctions in various cell types. ABLIM3 is involved in several critical biological pathways, including Netrin-1 signaling and nervous system development. Furthermore, it plays a fundamental role in embryonic development, cell lineage determination, and is linked to the progression of various cancers. Key interactions with proteins such as IKZF3, SSX2IP, MCM7, and GRB2 have been confirmed through advanced methods like yeast two-hybrid, co-IP, and pull-down assays.
ABLIM3 Analysis Services at Creative Biolabs
Creative Biolabs offers a comprehensive suite of analytical services, leveraging a proprietary collection of high-quality antibodies and cutting-edge techniques. Our expertise in ABLIM3 detection across human, mouse, and rat samples ensures accurate and reproducible results. We provide targeted analysis across several key areas to accelerate your research, including but not limited to the following:
- Gene and Chromosome Analysis
We provide in-depth analysis of the ABLIM3 gene's exon-intron structure and its precise chromosomal localization, providing foundational genetic context for your research.
- Protein Structure & Evolution
Our services include a detailed analysis of the ABLIM3 protein's structure and its phylogenesis, helping you understand its evolutionary history and functional domains.
- Subcellular Localization
We predict where ABLIM3 is located within the cell, offering critical insights into its functional context and interaction with other cellular components.
- In Silico Expression Analysis
Through computational methods, we analyze the expression of ABLIM3 across various tissue types and developmental stages to identify its physiological roles.
- Protein Interaction Mapping
We perform detailed mapping of ABLIM3-protein interactions to uncover new binding partners and pathways, crucial for understanding its molecular network.
- Quantitative Measurement
Our quantitative analysis services, including qPCR and mass spectrometry, provide precise and reproducible measurements of ABLIM3 expression levels.
Service Workflow
Our ABLIM3 analysis is a streamlined, step-by-step process designed for maximum efficiency and clarity.
01Initial Consultation & Project Design
We begin with a personalized discussion to understand your specific research goals and requirements for ABLIM3 analysis. This initial conversation allows us to craft a custom service plan that perfectly aligns with your project objectives.
02Sample Submission
You will provide us with your starting materials, such as cell lysates or tissue samples. We offer precise guidelines for sample quantity and preparation to ensure our ABLIM3 analysis yields optimal and reproducible results.
03Laboratory Analysis
Our expert team performs the requested ABLIM3 analyses using our state-of-the-art laboratory platforms and validated protocols. A rigorous quality control process is integrated at every stage to ensure data integrity and reproducibility.
04Final Data Delivery
You will receive a comprehensive report detailing the methods, raw data, and a professional interpretation of the results. This final package provides you with actionable insights to accelerate your research and discovery.
Applications
Cancer Biomarker Discovery
Neurodevelopmental Research
Given its involvement in Netrin-1 signaling and nervous system development, ABLIM3 analysis is key to understanding and identifying the molecular mechanisms underlying neurological disorders and neuronal migration defects.
Musculoskeletal Disease Studies
Precise analysis of ABLIM3 expression and function can provide insights into its role in cytoskeletal organization, which is fundamental to understanding muscle integrity and the pathology of musculoskeletal diseases.
Embryonic Development
Studying ABLIM3 is essential for deciphering its function in embryonic development and cell lineage determination, offering a window into the foundational processes of life and the origins of developmental disorders.
Service Highlights
- Exceptional Accuracy and Sensitivity: Our advanced methods and validated reagents ensure highly accurate and sensitive detection, guaranteeing reliable and reproducible results.
- Tailored Project Design: We collaborate with you to create a customized analysis plan that aligns perfectly with your research objectives, ensuring an efficient and focused approach.
- Expert Scientific Support: Our team of experienced biologists and data scientists offers comprehensive support throughout the entire process, from consultation to data interpretation.
FAQs
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What types of samples can you analyze for ABLIM3 expression?
We are capable of analyzing a wide range of biological samples for ABLIM3 expression. Our services have been optimized to work with cell lysates, tissue homogenates, and various cell culture materials. We routinely handle samples from human, mouse, and rat sources.
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How do you ensure the specificity of your ABLIM3 antibodies?
We utilize a rigorous validation process for all of our ABLIM3 antibodies. This includes confirming specificity through Western blotting with recombinant ABLIM3 protein and performing cross-reactivity checks with related proteins. Our antibodies are also verified in relevant biological models.
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Can you perform ABLIM3 analysis in a high-throughput manner for large-scale studies?
Yes, we can certainly perform ABLIM3 analysis in a high-throughput manner. Our platform is well-suited for large-scale biomarker screening projects. We have established protocols for efficient processing of numerous samples, ensuring consistency across all batches.
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What is the minimum amount of starting material required for your services?
The minimum amount of starting material required for a project depends on the specific analytical technique being employed. We will provide precise guidelines for sample quantity and preparation during our consultation. This ensures that we have sufficient material to conduct all requested assays effectively.
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How do you handle ABLIM3 protein-protein interaction studies?
Our protein-protein interaction studies for ABLIM3 are comprehensive. We use a combination of established methods such as co-immunoprecipitation and yeast two-hybrid assays. These techniques are complemented by advanced mass spectrometry to identify and validate ABLIM3's binding partners.
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Can your services help me identify post-translational modifications of ABLIM3?
Yes, our services are designed to identify post-translational modifications on ABLIM3. We specialize in phosphoproteomics and other advanced mass spectrometry techniques. These methods allow us to accurately pinpoint phosphorylation sites and other modifications that influence the protein's function.
Ready to take your biomarker research to the next level? Get in contact with our experts - We are happy to answer any question! Please feel free to contact us for more information.
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