Which type of analysis is better for my research: a qualitative WB or a quantitative ELISA?

The choice between a qualitative WB and a quantitative ELISA depends on your research goals. A Western Blot is ideal for determining the presence of the ADAMTS1 protein and its approximate size, while an ELISA is better for precisely quantifying the concentration of the protein in a sample, which is essential for biomarker studies where specific levels are critical for diagnosis or prognosis.

Is it possible to analyze ADAMTS1 activity rather than just its expression or concentration?

Yes, it is absolutely possible to analyze ADAMTS1's enzymatic activity. While gene expression and protein concentration are important, they don't always correlate with functional activity. We provide specialized assays that directly measure the enzymatic activity of ADAMTS1 by its ability to cleave specific substrates, giving you a complete understanding of its biological impact.

How do you ensure the specificity of your antibodies for ADAMTS1 in complex biological samples?

Our antibodies are meticulously validated for specificity to ADAMTS1. This includes rigorous testing using positive and negative controls, as well as cross-reactivity studies against other ADAMTS family members. This validation process ensures that our assays accurately detect ADAMTS1 without interference from related proteins, providing you with highly specific and reliable results.

Can you help with custom assay development if a standard ADAMTS1 kit is not suitable for my research needs?

Yes, we specialize in custom assay development. If your research requires a unique sample type, a different analytical platform, or a specific modification to a standard protocol, our team of experts can design, develop, and validate a bespoke ADAMTS1 assay to meet your exact specifications, providing a tailored solution that fits your unique research goals.

What are the different types of ADAMTS1 analysis you offer at the genetic level?

At the genetic level, we offer several types of ADAMTS1 analysis, including RT-PCR for gene expression quantification and mutation analysis to identify genetic variations. We also provide specialized services such as DNA methylation analysis, which is crucial for understanding epigenetic regulation and its role in disease development.

ADAMTS1 Analysis Service - Creative Biolabs

As a trusted global CRO company, Creative Biolabs' scientists are dedicated to helping you unlock the full potential of your research. We offer tailored ADAMTS1 analysis services, whether as part of an integrated pre-clinical package or as a stand-alone analytical option. We provide expert guidance to ensure you select the optimal strategy for your ADAMTS1 research, accelerating your path to discovery.

Introduction of ADAMTS1

ADAMTS1, A disintegrin and metalloproteinase with thrombospondin motifs 1, is a complex enzyme that serves as a highly sensitive biomarker for the early detection of various cancers, including prostate cancer (PC), hepatocellular carcinoma (HCC), and breast cancer (BC). It is synthesized as a prozymogen and undergoes N-linked glycosylation after protein translation. Its structure is characterized by three thrombospondin (TSP)-like domains at its C-terminus, which are crucial for its function.

ADAMTS1 functions in ECM remodeling by proteolytically degrading key substrates, such as collagen and versican. Its dysregulation can lead to pathological changes in the ECM and vascular density, highlighting its functional role in tumorigenic transformation. Research has identified ADAMTS1 DNA methylation in circulating cell-free DNA (cfDNA) as a promising blood biomarker for early pancreatic cancer detection, enabling a non-invasive diagnostic method. Additionally, studies suggest that serum ADAMTS1 levels could serve as a potential diagnostic marker for type A acute aortic dissection (AAD), expanding its relevance to cardiovascular health.

ADAMTS1 Analysis Services at Creative Biolabs

Creative Biolabs offers a comprehensive suite of accurate and cost-effective molecular services, available as both stand-alone options and integrated solutions. Our services are tailored to your specific needs, with custom-designed panels and data analysis packages available to support your unique research goals. Our immunoassay and genomics services are performed to the highest quality standards, ensuring that your biomarker data is robust, reliable, and accurate, helping you drive your research forward with confidence.

Our ADAMTS1 Analysis Service includes, but is not limited to, a range of specialized analyses:

We provide highly accurate quantification of ADAMTS1 gene expression, enabling you to precisely measure transcription levels in your samples. This is crucial for studying gene regulation and its impact on disease.

Our protein-level analysis suite includes ELISA for precise protein quantification, WB for detecting protein presence and size, and ISH/IF to visualize protein localization within tissues and cells.

Our comprehensive mutation analysis identifies genetic variations in the ADAMTS1 gene. Understanding these mutations is essential as they can impact protein function or disease susceptibility.

We perform epigenetic analysis to determine the methylation status of the ADAMTS1 gene promoter. This reveals how gene expression may be silenced without altering the DNA sequence itself.

Service Workflow

Our service workflow is a seamless, step-by-step process designed for maximum efficiency and collaboration.

01Initial Consultation & Project Design

The process begins with a detailed initial consultation with our expert scientists. We'll work closely with you to understand your specific research goals and design a customized ADAMTS1 analysis strategy.

02Sample Submission

After the project is designed, you submit your starting materials, which can include a variety of biological samples like cell lines, tissue samples, or biological fluids. Our team provides clear guidelines for proper collection and transport.

03Advanced Sample Processing

Upon receiving your samples, our team processes them using state-of-the-art technologies. We perform all necessary preparatory steps for both gene expression and protein analysis, adhering to strict quality control protocols.

04Comprehensive Analysis & Data Generation

We then conduct the agreed-upon ADAMTS1 analysis, which may include RT-PCR, ELISA, WB, or other specialized assays. Throughout this stage, we collect and generate robust, high-quality data from every experiment.

05Final Data Delivery & Report

The final step is the delivery of a comprehensive report. This includes all raw data, a detailed explanation of the methodologies used, and an in-depth analysis of the results to help you interpret the data and move your research forward.

Applications

Cancer Biomarker Discovery

ADAMTS1's altered expression and activity are implicated in multiple cancers, including prostate, breast, and hepatocellular carcinoma. Our analysis services can assist in validating ADAMTS1 as a diagnostic or prognostic biomarker, crucial for personalized medicine.

Angiogenesis Research

ADAMTS1 plays a dualistic role in regulating new blood vessel formation. Precise analysis of its levels and activity can provide critical insights into pro-angiogenic or anti-angiogenic pathways, which is vital for cancer and cardiovascular research.

Inflammatory Disease Studies

As a modulator of inflammatory responses, ADAMTS1 is a key target in research on autoimmune and inflammatory conditions like arthritis. Our services can help researchers quantify ADAMTS1 in inflammatory contexts to identify potential therapeutic targets.

Cardiovascular Disease Diagnosis

The link between serum ADAMTS1 levels and acute aortic dissection demonstrates its potential as a diagnostic marker. ADAMTS1 analysis can contribute to early detection and risk stratification in cardiovascular health.

Service Highlights

FAQs

Creative Biolabs also provides highly sensitive, custom-defined multiplexing options for the measurement of a range of analytes. Services are offered as an integrated service end-point or as a stand-alone option. If you do not see your desired ADAMTS1 service type mentioned above, please don't hesitate to contact us today to develop custom solution for specific needs. We will work to accommodate your request.