ADAMTS18 Analysis Service

Robust analysis of study samples is highly important to optimize therapeutic benefits and discover biomarkers. ADAMTS18 is a member of a disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS) family of proteases. At present, Creative Biolabs accelerates your ADAMTS18 research from hypothesis to candidate validation! Our biomarker services can be offered as part of an integrated pre-clinical package or as stand-alone analytical options.

Introduction of ADAMTS18

ADAMTS18 is a multifunctional protein known to play critical roles in development, angiogenesis, and coagulation. Dysregulation and mutation of ADAMTS18 have been implicated in the pathogenesis of numerous diseases, including inflammation, arthritis, atherosclerosis, and tumors. The structure of ADAMTS18 is complex, featuring a signal peptide, a pro-domain, a metalloprotease domain, a disintegrin domain, a central TS-1 domain, a Cys-rich domain, a spacer domain, and a TS-1-like repeat domain.

Fig.1 ADAMTS18 structure.¹

Researchers use ADAMTS18 knockdown models and specific antibodies to analyze its function, with binding confirmed by ELISA and WB. Epigenetic studies have identified ADAMTS18 as a tumor suppressor frequently silenced in human carcinomas. The loss of expression, genetic mutation, and gene methylation are all pathways that can lead to ADAMTS18 dysfunction and contribute to disease development.

Fig.2 Roles of ADAMTS18 in various pathophysiological conditions.

ADAMTS18 Analysis Services at Creative Biolabs

Our proven technology has advanced scientific research worldwide, providing critical insights and enabling thousands of researchers from biopharma and academia to make novel discoveries. Creative Biolabs offers comprehensive ADAMTS18 analysis services using multiple validated antibodies targeting human ADAMTS18. These antibodies are suitable for several applications, including WB, IHC, ICC, and sandwich ELISA, providing the robust data necessary to move your projects forward.

Our comprehensive services include, but are not limited to, the following key analyses:

• RT-PCR and ISH

For precise gene expression analysis and to detect gene silencing or dysregulation at the transcriptional level.

• ELISA and WB

To accurately detect and quantify ADAMTS18 protein levels in various sample types, from cell lysates to tissue sections.

• Prediction Analysis

To identify ADAMTS18 gene and protein interactions, offering a deeper understanding of its biological pathways.

• ADAMTS18-deficient Model Development

For in vivo studies to investigate the protein's function and role in disease.

• ADAMTS18 Mutation Analysis

To precisely characterize genetic variations associated with disease pathogenesis.

Service Workflow

Our ADAMTS18 analysis service workflow is a streamlined process designed for efficiency and collaboration.

Applications

Service Highlights

• Scientific Expertise: Our team consists of highly experienced biologists with deep knowledge of the ADAMTS family of proteases, ensuring a high level of scientific rigor in every project. We understand the nuances of ADAMTS18 analysis.
• Comprehensive Analytical Suite: We offer a full spectrum of services, from gene expression analysis to protein quantification, providing a complete solution for all your ADAMTS18 research needs.
• Customization and Flexibility: Our services are highly adaptable to the unique requirements of your research project, allowing for the development of custom protocols and models, such as ADAMTS18-deficient models.
• High-Quality, Reliable Data: We are committed to generating the most accurate and reproducible data possible. Our strict quality control measures ensure that the insights you receive are reliable and robust, providing a solid foundation for your discoveries.

FAQs

1. Can you perform both gene expression and protein level analysis of ADAMTS18 from a single sample?

   Yes, we are fully capable of performing both gene expression analysis using RT-PCR and protein level analysis using WB or ELISA from the same sample. This integrated approach provides a more comprehensive view of ADAMTS18 regulation.

2. Do you offer custom antibody development for ADAMTS18 or its specific isoforms?

   Yes, we offer custom antibody development services for ADAMTS18 and its specific isoforms to meet your unique research needs. Our team can work with you to design and generate highly specific antibodies for your applications.

3. How do you ensure the quality and accuracy of the ADAMTS18 analysis?

   We ensure quality and accuracy by using validated reagents, following strict, optimized protocols, and implementing rigorous quality control measures at every stage of the process. All data is reviewed by our expert team.

4. Can your services be used for pre-clinical drug development studies?

   Our ADAMTS18 analysis services are well-suited for pre-clinical drug development studies. We can assist in evaluating the efficacy of drug candidates by measuring their effects on ADAMTS18 expression and function.

5. What is the benefit of using an ADAMTS18-deficient model in my research?

   The use of an ADAMTS18-deficient model allows you to directly investigate the function of the protein in vivo and understand its role in a specific disease context. This model provides valuable insights into the protein's biological imperative.

6. Do you offer services for epigenetic analysis, such as gene methylation studies?

   Yes, we have the capabilities to perform epigenetic analysis, including gene methylation studies, which are crucial for understanding the silencing of the ADAMTS18 gene. This service provides a deeper level of insight into its regulatory mechanisms.

If you cannot find the service you're looking for, contact us today to develop custom solution for specific needs at Creative Biolabs.

Reference

1. Kumar, Saran, Nithya Rao, and Ruowen Ge. "Emerging roles of ADAMTSs in angiogenesis and cancer." Cancers 4.4 (2012): 1252-1299. Distributed under Open Access license CC BY 3.0. The image was modified by extracting and using only part of the original image. https://doi.org/10.3390/cancers4041252

For Research Use Only.