Creative Biolabs is a leading service provider that focuses on IVD (in vitro diagnostics) antibody development. Based on our extensive experience and advanced platform, now we are able to provide the antibody pairs development services for our customers all over the world. Our multifarious services can be tailor-designed to meet your specific needs.
Antibody pairs are two different antibodies generated against different epitopes or binding regions, but with the same target. This unique property allows the simultaneous binding to the same target against two different epitopes, which present great potential in biological research. Creative Biolabs now can develop the most suitable matched antibody pairs for our customers.
Fig.1 Antibody pairs.1, 4
An antibody pair always includes a capture antibody and a detection antibody which are the basis of many biological experiments, including but not limited to sandwich ELISA, IP-WB, and protein-protein interaction. The available antibody pairing formats can be two monoclonals (mAb/mAb), two polyclonals (pAb/pAb), or a combination (mAb/pAb) of both. It is worth noting that each antibody type offers distinct advantages, so it is necessary to appreciate the difference and how to use them in different conditions. Monoclonal antibodies can be spread used in all types of ELISAs and also work with polyclonal antibody to improve the chance of capturing antigen from a complex solution. Polyclonal antibodies present all the available epitopes in any given antigen but must be tested and validated thoroughly.
Fig.2 Applications of antibody pairs.
Moreover, the research of pharmacokinetics and pharmacodynamics in the field of the drug development is also dependent upon antibody pairs to some extent.
In order to avoid the time-consuming stage of antibody pair identification in sandwich ELISA, Creative Biolabs has developed the bead-based method for antibody pairs screening. The standard protocol can be divided into three main steps:
Moreover, we can also provide abundant matched antibody pairs to free up your time in searching and testing the appropriate capture and detection antibodies.
In addition to the IVD antibody pairs development, Creative Biolabs provides other services related to IVD antibody development which including monoclonal antibody development, polyclonal antibody development, and recombinant antibody development. With our well-established platform, the experienced scientists here at Creative Biolabs is dedicated to helping you with your special projects. If you are interested in our services, please do not hesitate to contact us for more details.
1. Monoclonal Antibody Pairs Against SARS-CoV-2 for Rapid Antigen Test
Fig.3 Binding of SARS-CoV-2 S1 and N mAbs for rapid antigen tests.2, 4
In this paper, researchers demonstrated rapid antigen tests that utilized murine-derived mAbs pairs conjugated to gold nanoparticles for detecting SARS-CoV-2, with minimal cross-reactivity to SARS-CoV-1 and other coronaviruses. They established a robust antibody screening approach to select mAb pairs targeting SARS-CoV-2 nucleocapsid (N) and spike (S) proteins. Linear epitope mapping of the mAbs revealed key interaction regions for lateral flow detection. The SARS-CoV-2 N antigen rapid test was validated using nasal swab samples, with results verified positive or negative by RT-PCR. Results were captured via mobile phone images, and signal intensities were adversely associated with RT-PCR cycle threshold (Ct) values. Overall, the developed rapid antigen tests provide an alternative tool for large-scale public health surveillance of COVID-19.
2. Development of Logic-Gated Antibody Pairs Acted on Co-Expressing Two Antigens
Fig.4 Design of Fc domain engineered IgG antibody pairs.3, 4
Building on previous findings, the researchers demonstrated that mAbs targeting different membrane receptors could hetero-oligomerize upon antigen binding, leading to synergistic CDC of tumor B cells. Then, the same study teams presented a general strategy to engineer IgG antibody pairs that triggered oligomerization-dependent functions only when both antibodies bound to the same target cell. This approach decouples functional activation from individual binding, allowing the antibody pairs to function as Boolean logic AND gates, integrating two binding signals into a functional outcome exclusively on cells or surfaces co-expressing both targets.
References
For Research Use Only.