Creative Biolabs understands the need for precise, intuitive diagnostic assays. We apply diverse bioconjugations like fluorescence labeling, protein-particle, and antibody-oligonucleotide conjugation. As a pioneer in in vitro diagnostics (IVD) and life sciences, we offer comprehensive, tailored conjugation services. These are available as part of IVD project development or as standalone solutions for our global clients. Our experienced scientists provide custom conjugation of various labels—HRP, biotin, fluorophores— either to an antibody selected from our extensive catalog or to customer-supplied biomolecules.
Introduction to Antibody Conjugation
Conventionally, molecular conjugation primarily involved labeling monoclonal or polyclonal antibodies with fluorescent dyes through either chemical or non-chemical methods, predominantly for detection in assays. However, with significant technological advancements, the landscape of bioconjugation has dramatically expanded. Today, an ever-increasing range of antibodies and other proteins can be precisely linked with an array of biologically active molecules. These include oligonucleotides and their sophisticated analogs, diverse peptides, other proteins, or virtually any molecular group capable of imparting valuable new properties. This encompasses critical elements such as potent radionuclides for imaging or therapy, catalytic enzymes, highly selective toxins for targeted drug delivery, robust metal chelates, versatile haptens for detection, and a vast selection of cutting-edge fluorophores for advanced microscopy and flow cytometry, thereby opening vast new avenues in biomedical research and diagnostics.
Fig.1 Antibody conjugates with different materials.1
Conjugation Services Provided by Creative Biolabs
Creative Biolabs proudly offers a comprehensive suite of custom conjugation services, designed to transform your biological components into powerful research tools. Our extensive range of services covers virtually all biomolecules and applications, leveraging our state-of-the-art technology platforms and the profound expertise of our scientific team. We provide tailored solutions, from initial consultation through meticulous execution and rigorous quality control, ensuring your project's success.
The conjugation services we can offer include but are not limited to:
 Protein Conjugation
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Various protein conjugations have been developed, such as glycoproteins, chromoproteins, enzyme-linked proteins, nucleoproteins, etc., for different in vitro diagnostic immunoassays and immunotherapy development. Creative Biolabs provides high-quality protein conjugation services for various biological applications with numerous materials. |
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Particle Conjugation
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Different kinds of multifunctional particles have been developed for various biological applications, such as biomolecule separation, cellular and molecular detection, drug delivery, etc. Creative Biolabs provides a number of particle conjugation services for molecular imaging, target identification, targeted drug delivery, and other related IVD applications. |
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Biotin Labeling
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Biotin-labeled antibodies, proteins, and nucleic acids have been widely used in numerous detection systems especially when the target of interest is expressed at low levels and cannot be detected easily. Experienced scientists at Creative Biolabs offer a full range of custom biotin labeling services without altering the bioactivity of target molecules. |
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Fluorescent Dyes Labeling
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Fluorescent dye labeling makes in vitro diagnostic immunoassays more convenient and intuitive. Creative Biolabs can conjugate your antibody or protein to numerous fluorescent dyes for the analysis of protein interactions, enzyme activity, conformational changes, protein localization, etc. Moreover, multiple fluorescence labeling is also within our capabilities. |
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Oligonucleotide Conjugation
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Oligonucleotide conjugations, including oligonucleotide-antibody, oligonucleotide-protein, and oligonucleotide-oligonucleotide, have been developed past few years in numerous applications from diagnostics to therapeutics. Added by state-of-the-art platforms, Creative Biolabs provides oligonucleotide conjugation services as a sensitive method for different IVD applications. |
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Special Labeling
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Electrochemiluminescent materials, especially Europium and Ruthenium, have also been applied for IVD immunoassays. These special conjugations have a longer fluorescence lifetime and perform well in temporal and spatial control. Creative Biolabs here also provides tailored special labeling services based on rich experience in bioconjugations. |
Custom Conjugation Project Workflow
We begin with an in-depth discussion to fully grasp your research objectives, the nature of your biomolecules, and the intended application. This crucial step guides the selection of optimal chemistry, linkers, and labels.
Our team develops a detailed experimental plan, including reaction conditions and purification strategies. This ensures the integrity and activity of both your starting material and the final conjugate are maintained, receiving your approval before execution.
Skilled chemists and biologists execute the reactions under controlled conditions to achieve precise labeling. Rigorous purification follows, effectively removing unconjugated labels and byproducts to ensure a clean product.
Each conjugate undergoes extensive analytical testing. Utilizing advanced techniques, we confirm identity, purity, concentration, and functional integrity, ensuring the conjugate meets our stringent quality standards.
The high-quality custom conjugate is delivered, accompanied by a comprehensive data package. This report details all analytical results and methodologies, providing complete transparency and supporting your downstream applications.
Features of Our Services
Diverse Selection of Labels
We offer an exceptionally wide array of labels, including an extensive spectrum of fluorescent dyes, enzymes, biotin, various small molecule drugs, and unique metal isotopes. This broad selection empowers researchers to choose the perfect tag for any downstream application, from high-sensitivity imaging to targeted therapeutics.
Experienced Scientific Team
Our professional team comprises highly skilled scientists with decades of specialized experience in bioconjugation chemistry and biomolecular engineering. Their profound knowledge ensures that even the most complex conjugation challenges are approached with innovative solutions and meticulous execution, leading to superior quality products.
Quality-First Customization
Our enterprise values prioritize quality and specific customization above all else. Every project benefits from a bespoke approach, where conjugation strategies are precisely tailored to preserve the biological activity and structural integrity of your unique biomolecules, guaranteeing a high-performing and stable conjugate.
Reproducible and Versatile Outcomes
The conjugation methodologies we employ are rigorously optimized for reproducibility, ensuring consistent results across batches. Furthermore, our robust processes are highly adaptable and applicable to a wide range of antibodies and proteins, facilitating seamless integration into diverse experimental designs and future scale-up.
Competitive Pricing & Expedited Turnaround
We are committed to providing highly competitive pricing structures without compromising on quality. Coupled with our optimized workflows and efficient project management, we consistently deliver conjugated products with remarkably fast turnaround times, helping you accelerate your research timelines and achieve quicker milestones.
Q&A
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Q: How do you ensure the functional activity of the biomolecules after conjugation?
A: Preserving functional activity is paramount in our conjugation process. We meticulously select the most appropriate linker chemistry and optimize reaction conditions to minimize alteration to the biomolecule's active sites. Following conjugation, rigorous functional assays, specifically designed for your molecule's intended application, are performed to confirm that its biological activity is fully retained.
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Q: Can I provide my own antibody or label for a custom conjugation project?
A: Absolutely. We frequently work with customer-supplied materials. Whether you have a proprietary antibody, a unique peptide, or a specialized label, our team will meticulously assess its suitability for conjugation. We conduct thorough incoming QC on all client-provided materials to ensure their quality before proceeding with the conjugation process.
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Q: How do you determine the optimal label-to-protein ratio (LPR) or Drug-to-Antibody Ratio (DAR) for a conjugate?
A: Determining the precise LPR or DAR is critical for conjugate performance. We employ advanced analytical techniques such as UV-Vis spectroscopy, mass spectrometry, and ICP-MS (for metal tags) to accurately quantify the label incorporation. Through careful optimization of reaction stoichiometry and purification, we ensure the desired and consistent ratio is achieved for each batch.
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Q: What specific QC analyses are performed on the finished conjugates?
A: Our comprehensive QC process is designed for uncompromising quality. Finished conjugates undergo a battery of analytical tests, including SEC-HPLC for purity and aggregation, SDS-PAGE for structural integrity, UV-Vis spectroscopy for concentration, and LPR/DAR, and often mass spectrometry for confirmation of conjugation sites. We also perform endotoxin testing and sterility checks for in vivo applications.
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Q: Can you assist with the selection of the most suitable linker chemistry for my specific application?
A: Definitely. Linker chemistry selection is a cornerstone of successful conjugation. Our scientific team, drawing from extensive experience, will guide you through the pros and cons of various linker types—cleavable vs. non-cleavable, site-specific vs. random—based on your target, payload, and desired biological fate, ensuring optimal performance and stability for your specific application.
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Q: What purification and characterization services are included post-conjugation?
A: Following conjugation, extensive purification is standard to remove unconjugated labels and reactants, often employing techniques like size exclusion chromatography (SEC), dialysis, or tangential flow filtration. Our characterization goes beyond basic purity, including comprehensive analysis of conjugation efficiency, molecular weight confirmation, and assessment of biological activity relevant to your downstream applications.
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Q: Are there specific considerations for conjugating highly sensitive or low-abundance biomolecules?
A: Conjugating sensitive or low-abundance biomolecules presents unique challenges, which our team is well-versed in overcoming. We employ mild reaction conditions, specialized low-concentration protocols, and carefully optimized purification methods to minimize sample loss and preserve the integrity and activity of delicate materials, ensuring successful outcomes even with challenging inputs.
As a pioneer company in IVD antibody development, Creative Biolabs is dedicated to offering different kinds of conjugation services to global customers for IVD immunoassays. Our dazzling array of conjugation services can be performed in-house as an extension of our antibody development services or as a stand-alone service. Don't hesitate to contact us, and discuss with our technical support about your conjugation needs.
Reference
- Zhou, Qun. "Site-specific antibody conjugation with payloads beyond cytotoxins." Molecules 28.3 (2023): 917. Distributed under Open Access license CC BY 4.0, without modification.
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