PCR based Kits

In the dynamic and rapidly evolving field of molecular biology, the polymerase chain reaction (PCR) has emerged as a revolutionary technique, fundamentally transforming how scientists study DNA. Our PCR-based kits are at the forefront of this innovation, meticulously engineered to empower researchers across diverse disciplines, offering a reliable, efficient, and user-friendly solution to unlock the secrets hidden within DNA.

Key Principles

Leveraging cutting-edge PCR technology, the kits incorporate specifically designed primers and fluorescent probes targeting the highly conserved regions of the target gene. During the PCR reaction, these primers selectively bind to the target DNA region, initiating DNA replication and amplification. The fluorescent probes then hybridize with specific sequences within the amplified products, enabling enzymatic cleavage, thereby releasing a detectable fluorescent signal. By continuously monitoring changes in this fluorescent signal, the presence or absence of target DNA in the sample can be accurately determined, confirming whether the sample contains the target component.

PCR-based kits. (Creative Biolabs Authorized)

Diverse Applications

Pathogen Detection

PCR-based kits can rapidly and accurately detect pathogens like viruses and bacteria. By amplifying specific DNA/RNA sequences of the pathogens, these kits enable early identification.

Genetic Disorder Screening

In the field of genetics, PCR-based kits play a crucial role in screening for genetic disorders. They can amplify target genes associated with conditions such as cystic fibrosis or sickle cell anemia.

Gene Expression Quantification

RT-qPCR kits enable precise measurement of mRNA levels by combining reverse transcription and real-time amplification. Widely used in developmental biology, they track dynamic gene expression changes during embryogenesis or tissue differentiation.

Cancer Research

In cancer research, PCR-based kits assist in studying gene expression patterns and identifying cancer biomarkers. By amplifying cancer-related genes, researchers can understand tumor development mechanisms, predict disease prognosis, and discover potential targets.

Core Features

Ready-to-Use Convenience

We know time is crucial in research and diagnostics. Our kit is designed for maximum ease; users only need to provide the sample DNA template. No complex preparation steps are required, enabling you to focus on getting accurate results fast.

High-Sensitivity with Optimized Primers and Probes

Through extensive R&D, our primers and probes are optimized to offer outstanding sensitivity. This high sensitivity ensures that even trace amounts of the target are identified, suitable for low-abundance samples or early-stage infection detection.

Positive Control for Reliable Findings

False-negative results are a concern in molecular diagnostics. Our kit includes a positive control as a benchmark. It helps you easily distinguish true negative samples from potential false negatives due to sample issues or improper handling, giving you confidence in result accuracy.

Exceptional Specificity for Precise Identification

Specificity is key to our kit's performance. Primers are designed based on the highly conserved regions of the target DNA, preventing cross-reaction with other DNA sequences. This ensures the detected fluorescent signal truly indicates target DNA presence, eliminating false positives from non-specific binding, making it ideal for accurate target identification applications.

FAQs

  1. Can I use my own primers with your PCR master mix?

    Yes, our PCR master mix is compatible with a wide range of primers. However, we recommend optimizing the primer concentration and annealing temperature for your specific application to ensure optimal performance. Our technical support team is also available to provide guidance and assistance if needed.

  2. How do I troubleshoot if I don't get the expected PCR results?

    If you encounter issues with your PCR results, there are several steps you can take to troubleshoot the problem. First, check the quality of your DNA sample to ensure that it is pure and free of contaminants. Next, verify that the reaction setup is correct, including the volumes of reagents and the addition of the sample DNA. Finally, check the thermal cycler settings to ensure that they are appropriate for your experiment.

  3. Are your PCR-based kits suitable for high-throughput applications?

    Absolutely! Our kits are designed to be scalable and can be easily adapted for high-throughput screening in large-scale research projects. The pre-formulated master mix and user-friendly design make it easy to set up multiple reactions simultaneously, saving time and effort in high-throughput applications.

Our PCR-based kits offer a powerful and versatile tool for molecular biology research and diagnostics. With their high sensitivity, specificity, speed, and user-friendly design, they are the ideal choice for unlocking the potential of DNA analysis. Explore our range of PCR-based kits today and take your research to the next level!

Product list

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      • PCR Based Kit Soybean Nucleic Acid Detection Kit (CAT#: Z01MM-0525-JL1)
        • Tag: PCR Based Kit
        • Subcategory: PCR Kit
        • Analyte: Soybean DNA
        • Target Source: Soybean
        • Species Reactivity: Soybean
      • PCR Based Kit Rat Nucleic Acid Detection Kit (CAT#: Z01MM-0525-JL2)
        • Tag: PCR Based Kit
        • Subcategory: PCR Kit
        • Analyte: Rat DNA
        • Target Source: Rat
        • Species Reactivity: Rat
      • PCR Based Kit Mammal Nucleic Acid Detection Kit (CAT#: Z01MM-0525-JL3)
        • Tag: PCR Based Kit
        • Subcategory: PCR Kit
        • Analyte: Mammal DNA
        • Target Source: Mammal
        • Species Reactivity: Mammal
      • PCR Based Kit Cordyceps Sinensis Nucleic Acid Detection Kit (CAT#: Z01MM-0525-JL4)
        • Tag: PCR Based Kit
        • Subcategory: PCR Kit
        • Analyte: Cordyceps Sinensis DNA
        • Target Source: Cordyceps Sinensis
        • Species Reactivity: Cordyceps Sinensis
      • PCR Based Kit Goose Nucleic Acid Detection Kit (CAT#: Z01MM-0525-JL5)
        • Tag: PCR Based Kit
        • Subcategory: PCR Kit
        • Analyte: Goose DNA
        • Target Source: Goose
        • Species Reactivity: Goose
      • PCR Based Kit Leopard Nucleic Acid Detection Kit (CAT#: Z01MM-0525-JL6)
        • Tag: PCR Based Kit
        • Subcategory: PCR Kit
        • Analyte: Leopard DNA
        • Target Source: Leopard
        • Species Reactivity: Leopard
      • PCR Based Kit Brazil Nut Nucleic Acid Detection Kit (CAT#: Z01MM-0525-JL7)
        • Tag: PCR Based Kit
        • Subcategory: PCR Kit
        • Analyte: Brazil Nut DNA
        • Target Source: Brazil Nut
        • Species Reactivity: Brazil Nut
      • PCR Based Kit Quail Nucleic Acid Detection Kit (CAT#: Z01MM-0525-JL8)
        • Tag: PCR Based Kit
        • Subcategory: PCR Kit
        • Analyte: Quail DNA
        • Target Source: Quail
        • Species Reactivity: Quail
      • PCR Based Kit Monkfish Nucleic Acid Detection Kit (CAT#: Z01MM-0525-JL9)
        • Tag: PCR Based Kit
        • Subcategory: PCR Kit
        • Analyte: Monkfish DNA
        • Target Source: Monkfish
        • Species Reactivity: Monkfish
      • PCR Based Kit Pistachio Nucleic Acid Detection Kit (CAT#: Z01MM-0525-JL10)
        • Tag: PCR Based Kit
        • Subcategory: PCR Kit
        • Analyte: Pistachio DNA
        • Target Source: Pistachio
        • Species Reactivity: Pistachio
      • PCR Based Kit Wolf Nucleic Acid Detection Kit (CAT#: Z01MM-0525-JL11)
        • Tag: PCR Based Kit
        • Subcategory: PCR Kit
        • Analyte: Wolf DNA
        • Target Source: Wolf
        • Species Reactivity: Wolf
      • PCR Based Kit Deer Nucleic Acid Detection Kit (CAT#: Z01MM-0525-JL12)
        • Tag: PCR Based Kit
        • Subcategory: PCR Kit
        • Analyte: Deer DNA
        • Target Source: Deer
        • Species Reactivity: Deer
      • PCR Based Kit Mule Nucleic Acid Detection Kit (CAT#: Z01MM-0525-JL13)
        • Tag: PCR Based Kit
        • Subcategory: PCR Kit
        • Analyte: Mule DNA
        • Target Source: Mule
        • Species Reactivity: Mule
      • PCR Based Kit Spirochemical Component Detection Kit (CAT#: Z01MM-0525-JL14)
        • Tag: PCR Based Kit
        • Subcategory: PCR Kit
        • Analyte: Spirochemical DNA
        • Target Source: Spirochemical
        • Species Reactivity: Spirochemical
      • PCR Based Kit Anoplopoma Fimbria Nucleic Acid Detection Kit (CAT#: Z01MM-0525-JL15)
        • Tag: PCR Based Kit
        • Subcategory: PCR Kit
        • Analyte: Anoplopoma Fimbria DNA
        • Target Source: Anoplopoma Fimbria
        • Species Reactivity: Anoplopoma Fimbria
      • PCR Based Kit Camel Nucleic Acid Detection Kit (CAT#: Z01MM-0525-JL16)
        • Tag: PCR Based Kit
        • Subcategory: PCR Kit
        • Analyte: Camel DNA
        • Target Source: Camel
        • Species Reactivity: Camel
      • PCR Based Kit Donkey Nucleic Acid Detection Kit (CAT#: Z01MM-0525-JL17)
        • Tag: PCR Based Kit
        • Subcategory: PCR Kit
        • Analyte: Donkey DNA
        • Target Source: Donkey
        • Species Reactivity: Donkey
      • PCR Based Kit Potato Nucleic Acid Detection Kit (CAT#: Z01MM-0525-JL18)
        • Tag: PCR Based Kit
        • Subcategory: PCR Kit
        • Analyte: Potato DNA
        • Target Source: Potato
        • Species Reactivity: Potato
      • PCR Based Kit Horse Nucleic Acid Detection Kit (CAT#: Z01MM-0525-JL19)
        • Tag: PCR Based Kit
        • Subcategory: PCR Kit
        • Analyte: Horse DNA
        • Target Source: Horse
        • Species Reactivity: Horse
      • PCR Based Kit Cat Nucleic Acid Detection Kit (CAT#: Z01MM-0525-JL20)
        • Tag: PCR Based Kit
        • Subcategory: PCR Kit
        • Analyte: Cat DNA
        • Target Source: Cat
        • Species Reactivity: Cat

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