CHI3L1 serves as a crucial biomarker for tumor progression and metastasis, offering critical insights into disease aggressiveness. Its analysis can help predict patient prognosis in various cancers and, notably, assess the potential response to targeted therapies and emerging immunotherapies. This provides a valuable tool for patient stratification and therapeutic guidance.
Creative Biolabs provides specialized CHI3L1 analysis services, offering a variety of strategic options to help clients accurately quantify and understand the role of this critical biomarker in their research. We empower you to make informed decisions for therapeutic and diagnostic development.
Introduction of CHI3L1
Chitinase-3-like protein 1 (CHI3L1), also known as YKL-40, is a 39 kDa secreted glycoprotein that plays a pivotal role in the body's inflammatory and tissue remodeling responses. Produced by cells such as activated macrophages and neutrophils, its presence is a significant indicator of an underlying biological process. Unlike true chitinases, CHI3L1 lacks enzymatic activity, functioning instead as a non-catalytic lectin that regulates cell proliferation, adhesion, and migration, making it a powerful target for analysis.
Studies have extensively demonstrated the clinical relevance of CHI3L1, revealing that its circulating levels are frequently elevated in a wide range of diseases. This includes many malignancies, such as glioblastoma, as well as cancers of the colon, breast, prostate, and kidney. Furthermore, its expression is a reliable marker for pathological conditions like fibrosis, with studies showing its ability to differentiate between early and late stages of liver fibrosis. CHI3L1 is also considered a potential biomarker for cystic fibrosis disease severity, highlighting its broad utility.
Fig.1 CHI3L1 promotes blood vessel formation and cancer cell growth, contributing to the advancement of glioblastoma.1
CHI3L1 Analysis Services at Creative Biolabs
Creative Biolabs provides comprehensive CHI3L1 analysis services, taking full advantage of our material resources and perfect assay solutions. We have developed CHI3L1 specific antibodies that can be used in different scientific applications, including WB, IHC, ICC, ELISA and FCM. Our services are designed to address the diverse needs of researchers and drug developers, from basic quantification to advanced functional studies.
Our analytical services include but are not limited to the following:
- High-Sensitivity ELISA
Quantitative measurement of serum, plasma, or cerebrospinal fluid (CSF) CHI3L1 levels.
- qRT-PCR
Detection and quantification of CHI3L1 mRNA expression.
- Indirect Immunohistochemistry & Immunocytochemistry
Visualization and localization of CHI3L1 expression in tissue IHC and cell confocal microscopic analyses.
- Functional Assays
Evaluation of the biological effects of CHI3L1 on cancer cells, and CHI3L1-knockdown cell line generation for in vitro apoptosis-related assays
- Custom Antibody Generation
Development of specific antibodies for unique research applications, including anti-CHI3L1 and humanized bispecific antibodies against CHI3L1 and PD-1
- Vector Generation
Creation of a truncated form of the human CHI3L1 expression vector for specific functional and expression studies.
Service Workflow
Our CHI3L1 analysis service is a streamlined, step-by-step process meticulously designed for maximum efficiency and clarity, ensuring a seamless and transparent experience from initial inquiry to final report delivery.
01Initial Consultation
We begin with a detailed and collaborative discussion to thoroughly understand your specific research goals and provide a customized project plan with a clear timeline for your CHI3L1 analysis, ensuring all requirements are met.
02Starting Materials Submission
The client provides the biological samples (e.g., serum, tissue, cell lines) necessary for the requested analysis. Our team offers guidance on proper sample preparation and secure shipping to ensure their integrity.
03Laboratory Processing
Our expert team conducts the requested assays, such as ELISA, qRT-PCR, or IHC, adhering to strict, validated protocols to ensure the highest data quality and reproducibility throughout the entire process.
04Data Delivery
We deliver a comprehensive final report containing all raw data, detailed methodologies, and professional interpretations of the CHI3L1 analysis results to directly support your research and facilitate your next steps with confidence.
Applications
Oncology
Inflammatory Diseases
In chronic inflammatory conditions, such as liver fibrosis, rheumatoid arthritis, and cystic fibrosis, CHI3L1 analysis provides a non-invasive and precise method for monitoring disease progression, evaluating the effectiveness of treatments, and guiding personalized therapeutic strategies. This enables clinicians to better track disease activity and optimize patient care.
Neurodegenerative Disorders
The protein's role in neuroinflammation makes it a valuable biomarker for diseases like Multiple Sclerosis (MS) and Alzheimer's disease (AD). It allows for the non-invasive and real-time monitoring of ongoing glial activation in the central nervous system, offering a crucial window into disease activity.
Service Highlights
- Scientific Expertise: Our team of biologists and specialists provides a high level of expertise in CHI3L1 analysis, ensuring reliable and accurate results.
- Cutting-Edge Technology: We utilize advanced analytical platforms, including high-sensitivity ELISA and advanced IHC, to provide precise quantification and visualization.
- Customization: We offer tailored analysis options to meet the unique needs of your specific research project, from singleplex assays to complex functional studies.
- Actionable Data: Our reports are designed to deliver clear, comprehensive, and interpretable data that can directly support your research decisions.
FAQs
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Can you analyze CHI3L1 expression in specific cell types within a tissue sample?
Yes, we are able to analyze CHI3L1 expression in specific cell types. We utilize advanced IHC techniques combined with multiplexed staining to visualize and quantify CHI3L1 protein localization within the context of a tissue sample, allowing for precise cellular-level analysis.
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Do you offer services to study the functional effects of CHI3L1?
We do offer services to study the functional effects of CHI3L1. These services include our specialized CHI3L1-knockdown cell line generation service for in vitro assays and the evaluation of its biological effects on cancer cell proliferation and apoptosis in a controlled laboratory setting.
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What are the starting material requirements for tissue analysis?
The starting material requirements for tissue analysis include properly fixed and embedded tissue blocks. We accept formalin-fixed paraffin-embedded (FFPE) tissue blocks, as these are the preferred format for our IHC and other tissue-based CHI3L1 expression studies.
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Can I combine CHI3L1 analysis with other biomarker services?
You can certainly combine CHI3L1 analysis with other biomarker services. We can perform multiplexed analysis that allows for the simultaneous detection and quantification of multiple biomarkers within a single sample, providing you with a more comprehensive overview of your research subject.
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What kind of data will I receive in the final report?
The final report you will receive will contain a comprehensive set of data. It will include all raw data from the analysis, detailed protocols and methodologies used during the assays, and a professional summary of the results with a clear interpretation of the findings for your project.
If you are interested in our analysis services at Creative Biolabs, please feel free to contact us for more information.
Reference
- Zhao, Ting, et al. "Chitinase-3 like-protein-1 function and its role in diseases." Signal transduction and targeted therapy 5.1 (2020): 201. Distributed under Open Access license CC BY 4.0, without modification. https://doi.org/10.1038/s41392-020-00303-7
For Research Use Only.
