Hydrogen Peroxide Electrochemical Biosensor

Hydrogen peroxide (H₂O₂) is an important molecule within the human body, but many of its roles in physiology and pathophysiology are not well understood. To better understand the importance of H₂O₂ in biological systems, it is essential that researchers are able to quantify H₂O₂ in various settings such as in vitro, ex vivo and in vivo systems.

Introduction

H₂O₂ is a reactive oxygen species (ROS) that is present throughout the body, playing various roles in physiological processes. At high levels, H₂O₂ can be detrimental to the body, resulting in cell damage, inflammatory disease, and even cancer. However, low physiological concentration of H₂O₂ makes accurate detection difficult. To improve the understanding of H₂O₂‘s role in biological systems, researchers have developed different sensors to detect and quantify H₂O₂ under various conditions. The two classes of sensors that are typically used for H₂O₂ detection are light detecting sensors and electrochemical sensors.

Electrochemical Sensors

Two types of electrochemical probes are frequently used for H₂O₂ detection, namely potentiometric and amperometric. The working principle of the two probes is different.

• Potentiometric probes measure the potential (voltage) between a working and reference electrode in a system that has no significant current flow. The working electrode needs to be modified so that changes in potential correlate to changes in H₂O₂ concentration, and the reference electrode must remain constant so that it can serve as a reference, or comparison, to the working electrode.
• Amperometric sensors rely on the principle that changes in current are correlated to change in concentration. Thus, amperometric sensors use two or three electrodes to measure the change in the current of a sample while the potential (voltage) is held constant.

Fig.1 Two different methods of electrochemical detection. (Meier, 2019)

In the electrochemical methods, different types of modified electrodes have been used. Horseradish peroxidase (HRP) enzyme is one of the mostly used materials for the modification of electrode. An schematic representation of the electrochemical design H₂O₂ biosensor was given in Figure 2.

Owing to the expensiveness and unstableness of HRP, finding alternatives to reduce the cost and to improve the performance of H₂O₂ biosensors is scientifically important. Hb can be utilized as the HRP substitute in the detection of H₂O₂ in virtue of the low cost and the stable property of in solution. Although Hb does not play a role as an electron transfer carrier in biological systems, it has been shown to possess enzyme-like catalytic activity.

Fig.2 The illustration of the electrochemical design H₂O₂ biosensor. (Cui, 2011)

With the development of nanoscience and nanotechnology, carbon nanotubes and gold nanoparticles (GNPs) are widely used with Hb for the construction of biosensor. The nanomaterial-based electrochemical H₂O₂ biosensor usually possesses a larger linear range and better sensitivity, and it is more applicable to industrial and environmental monitoring.

References

1. Meier, J.; et al. Hydrogen peroxide sensors for biomedical applications. Chemosensors. 2019, 7(4): 64.
2. Cui, L.; et al. A novel hydrogen peroxide biosensor based on the specific binding of horseradish peroxidase with polymeric thiophene-3-boronic acid monolayer in hydrophilic room temperature ionic liquid. Synthetic metals. 2011, 161(15-16): 1686-1690.

For Research Use Only.