In vitro diagnostic (IVD) antibodies have become critical components of many diagnostic assays. As a leading antibody service supplier, Creative Biolabs is specialized in the development of IVD antibodies against a variety of disease biomarkers with low cross-reactivity and high specificity. Aspartate aminotransferase (AST or AAT) is a promising biomarker with potential for liver fibrosis diagnosis. Here, we introduce our IVD antibody development services targeting AST marker.

Introduction of AST

AST, formerly known as SGOT (serum glutamic-oxaloacetic transaminase), is a pyridoxal phosphate (PLP)-dependent transaminase enzyme. AST is a homodimer that consists of two identical subunits, and each subunit is composed of a large and a small domain, connected by a long α-helix consisting of 32 amino acids. AST can catalyze the reversible transfer of an α-amino group between aspartate and glutamate, so it is an important enzyme in amino acid metabolism. AST shows more specificity to the liver but is also found in the heart, skeletal muscle, kidneys, brain, pancreas, and red blood cells. As its name refers to, the most well-known function of AST is to catalyze the interconversion of aspartate and α-ketoglutarate to oxaloacetate and glutamate to maintain the homeostasis in organisms.

Structure of AST from Corynebacterium glutamicum. Fig.1 Structure of AST from Corynebacterium glutamicum. (Son, H. F., 2016)

AST Marker for Liver Fibrosis

Hepatic fibrosis is a pathological process occurred in the liver. The levels of serum AST are indicative of tissue damage, especially in the liver. It has been reported that injury to hepatocytes or liver disease will cause leakage of AST into the extracellular compartment with a subsequent elevation in serum AST activity. Substantial hepatic necrosis as found in chemical and ischemic injury appears to be particularly associated with elevation of AST enzymes. Therefore, serum AST test combined with other enzymes to monitor the course of various liver disorders is a valuable aid primarily in the diagnosis of liver disease. The common detection methods include colorimetric, spectrophotometric, chemiluminescence, chromatography, fluorescence and UV absorbance, radiochemical, and electrochemical techniques, etc. AST has become a reliable and sensitive biomarker of liver disease and reflects the severity of liver fibrosis.

Mechanisms of hepatic fibrogenesis and possible molecular serum biomarkers. Fig.2 Mechanisms of hepatic fibrogenesis and possible molecular serum biomarkers. (Liu, T., 2012)

IVD Antibody Development Services for AST Marker

Because of the importance of AST in the diagnostic liver function tests, it is necessary to explore high-quality IVD antibodies against AST. Detection of serum AST may benefit the detection of liver fibrosis at an early stage. At Creative Biolabs, we offer a full range of antibody development, antigen & antibody conjugation, and IVD kit development services to clients across the globe. We develop antibodies that can be applied for different immunoassay formats, including ELISAs, lateral flow immunochromatographic assays, western blot, and flow cytometry.

Supported by our highly specialized scientists and advanced platform, we are confident in providing services with the best quality and the most competitive prices. If you are interested in discovering novel IVD antibody, please do not hesitate to contact us for more details.

References

  1. Son, H. F., (2016). “Structural Insights into a Novel Class of Aspartate Aminotransferase from Corynebacterium glutamicum.” PloS one, 11(6), e0158402.
  2. Liu, T., (2012). “Molecular serum markers of liver fibrosis.” Biomarker insights, 7, BMI-S10009.


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