Creative Biolabs is a world leader in the field of in vitro diagnostics (IVD) antibody development. With our extensive experience and advanced platform, we provide a range of IVD antibody development services against CD117 marker for the diagnosis of gastrointestinal cancer.
CD117
CD117, also known as proto-oncogene c-Kit, Mast/stem cell growth factor receptor (SCFR) or tyrosine-protein kinase kit, is a human receptor tyrosine kinase protein encoded by the KIT gene. CD117 is an important cell surface marker, a cytokine receptor expressed on the surface of hematopoietic stem cells and on other cell types. CD117 can be used to identify certain types of hematopoietic progenitors in the bone marrow. CD117 is a type III receptor tyrosine kinase that binds to stem cell factor, a substance that causes the growth of certain types of cells, also known as "steel factor" or "c-kit ligand". When this receptor binds to the stem cell factor (SCF), it forms a dimer which activates its intrinsic tyrosine kinase activity, which in turn phosphorylates and activates signal transduction molecules that propagate the signal in the cell. Signaling through CD117 is important in cell survival, proliferation, and differentiation. Thus, the altered form of CD117 receptors may be associated with some types of cancers.
Fig.1 Immunohistochemical features of GIST. (A. Spindle cell GIST with strong and diffuse cytoplasmic staining of CD117 (c-kit) (×400)). (Zhao, 2012)
CD117 as Marker of Gastrointestinal Cancer
Gastrointestinal stromal tumor (GIST) is the most common gastrointestinal mesenchymal tumor. GIST is derived from smooth muscle pacemaker interstitial cell of Cajal or similar cells. Histopathologists found it hard to tell the difference between the types of molecules decades ago. Subsequently, CD34 and later CD117 were identified as markers to distinguish the various types. Most of GISTs (66%) occur in the stomach and gastric GISTs have a lower malignant potential than tumors found elsewhere in the GI tract.
About 85% of GISTs are associated with abnormal c-KIT pathways. KIT is a gene encoding a transmembrane receptor for a growth factor. CD117, the product of c-KIT is expressed on ICCs and numerous other cells, predominantly melanocytes, mast cells, bone marrow cells, and several other cells. However, in the gut, CD117-positive mass is likely to be GIST produced by ICC cells.
Contrary to the causes of other similar tumors, pathologists can use the immunohistochemical, specific antibody to CD117, when GIST is suspected. 95% of all GISTs are CD117 positive. Other possible markers include DOG-1, CD34, vimentin, and desmin. Other cells showing positive for CD117 are mast cells. Creative Biolabs offers CD117-specific antibody (pair) development for in vitro diagnosis of gastrointestinal cancer. CD117 antibodies are used in qualified laboratories and identified the presence of associated antigens in sections of formalin-fixed, paraffin-embedded tissue sections using IHC test methods by light microscopy. For clinical differential diagnosis of gastrointestinal cancer, the use of this antibody is indicated as an approach of identifying gastrointestinal cancer in the context of antibody panels.
Creative Biolabs has focused on IVD antibody discovery for years. We have experts who are able to develop high-affinity IVD antibodies against varies of markers. We are therefore confident in offering the most suitable service and finest results for our customers all over the world. If you are interested in our IVD antibody discovery services, please contact us for more details.
Reference
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