Creative Biolabs is a contract research organization specialized in offering customized services for the development of antibodies for use in diagnostics, drug discovery, and basic research. Especially, we provide the expertise and personnel to offer high-quality in vitro diagnostic (IVD) antibody and immunoassay development services targeting a wide range of high-value diagnostic biomarkers to our clients. Here, we introduce our IVD antibody development services for the sex hormone-binding globulin (SHBG) marker.
SHBG is a glycoprotein produced by the liver that binds sex steroids (such as estrogen, dihydrotestosterone, and testosterone) in the circulation, regulating their availability and access to target organs. SHBG binds these sex hormones with distinct affinity, having a high affinity to dihydrotestosterone, medium affinity to testosterone and oestradiol, and only a low affinity to estrone. The plasma concentrations of SHBG are regulated by androgen/estrogen balance, thyroid hormones, insulin, and dietary factors.
Numerous studies have shown that SHBG levels are reduced in obesity, insulin resistance, metabolic syndrome, and type 2 diabetes, while elevated in diseases such as hepatitis, hyperthyroidism, HIV, etc. Thus, the level of SHBG may be a biomarker for different diseases. For instance, Tawfeek et al. (2017) identified circulating SHBG concentration as a potentially useful new biomarker for prediction of risk of gestational diabetes (GDM), which is a common pregnancy complication associated with increased maternal and neonatal morbidities. Xargay-Torrent et al. (2018) reported circulating SHBG to be an integrating biomarker for an adverse cardiometabolic profile in obese pregnant women. Cheng et al. (2018) reported that blood SHBG is a potential early diagnostic biomarker for gastric cancer.
Fig.1 Molecular mechanisms regulating hepatic SHBG production. (Simo, 2015)
The measurement of protein-based biomarkers is largely dependent on and carried out using immunoassays, due to their technical ease and low cost. These immunoassays apply analyte-specific antibody (pairs) to achieve high specificity and sensitivity. Different formats of immunoassays targeting SHBG have been reported, such as sandwich ELISA, Elecsys® chemiluminescence assay, and particle-enhanced turbidimetric immunoassay.
At Creative Biolabs, we have established a comprehensive IVD platform to offer unparalleled custom-specific IVD antibody development services to clients with IVD requirements. We also offer one-stop immunoassay development services for SHBG marker, providing antibody development, antibody conjugation, assay design, protocol establishment, optimization, and kit production services. We can also start with provided antibodies and conduct large-scale microplate coating services. For more information, please click the links below:
Creative Biolabs has successfully completed numerous IVD antibody generation and development projects for clients across the globe. We are confident that our commitment to science and research will enable us to offer you the best products and services. Contact us to discuss your project and experience the great value of our services.
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