Creative Biolabs is a well-known expert in in vitro diagnostics (IVD) market, including IVD antibody development platforms and customized services. Recently, there are microplates coating kits launched to determine disease diagnosis and monitor biochemical reactions. Meanwhile, we also can accomplish an application-specific coating process that suits client’s needs.

Microplates Coating

Normal microplates of polystyrene have been widely applied in immunoassays for the early detection, as well as monitoring biological or chemical responses, toxicity tests, combinatorial synthesis, cell growth, high-throughput screening, and so on. Typically, the biological molecules immobilized onto the microplate surface are performed through hydrophobic interactions. The fixation of various small ligands requires microplates with a pretreated surface of amino or carboxylic groups.

Coated Microplate.Fig.1 Coated Microplate. (Akbulut, M., 2011)

Currently, there is a new versatile approach for the modification of the well surfaces of microplates with poly N-phenylethylene diamine methacrylamide (Poly-NPEDMA). The chemical grafting of poly-NPEDMA to the microplate surface forms thin layers of a polyaniline derivative containing pendant methacrylamide double bonds. The attachment points are used for various functional polymers by photochemical grafting of polymers (e.g. acrylate and methacrylate) with different functionalities. The uniformity of coatings is confirmed spectrophotometrically via microscopic imaging and contact angle (CA) measurements. The feasibility of this technology has been displayed by the generation of a small library of polymers attached to the microplate surfaces and screening of their affinity to small molecules. The ease of preparation, stability of the polymers, reproducibility of measurement, and cost-effectiveness make this method suitable for high-throughput screening in the area of material research.

Microplates Coating Development

The microplates, however, have several drawbacks associated with the relatively low surface area, only supporting small quantities of ligands to be immobilized onto their surfaces. Thus, there is a need for improved microplates with a high density of different functional groups. Toward this end, Creative Biolabs can offer efficient and cost-effective microplate coating development services and related kits for researchers used in IVD diagnosis and prognosis determination. Our trained operators concentrate on microplate coating systems and quality control that makes sure our productions are precision and high-quality. Coated microplates are available in a host of fields, including immunological or nucleic acid hybridization assays. The microplates have a high affinity towards biotin, are suitable in any size of the formats and stable over a wide spectrum of pH, ionic strength, and detergent concentration. For more detailed information, please feel free to contact us.

Reference

  1. Akbulut, M., (2011). “Microplates with adaptive surfaces.” ACS Comb Sci, 13(6), 646-652.


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