Nicotinamide phosphoribosyltransferase (NAMPT) plays a key role in cell metabolism and is closely related to the occurrence and development of many cancers. Creative Biolabs has developed modular core assays which offer the flexibility to tailor assay content to meet individual project needs with a triad of custom solutions. We now provide custom NAMPT analysis services for global clients.
Background
NAMPT is a rate-limiting enzyme in the salvage pathway of nicotinamide adenine dinucleotide (NAD) biosynthesis. NAMPT exhibits dual functionality- as an intracellular enzyme which regulates nicotinamide adenine dinucleotide metabolism and as an extracellular protein (eNAMPT) secreted into the blood and functions as a cytokine regulator of innate immunity.
Fig.1 The roles of the extracellular and intracellular forms of NAMPT.1
NAMPT expression is significantly elevated in various human malignant tumors, such as gastric cancer, lymphomas, breast cancer, prostate adenocarcinoma, glioblastoma, colorectal adenocarcinoma, esophageal cancer, and ovarian serous adenocarcinoma. Higher NAMPT expression is associated with deeper tumor invasion, presence of lymph node metastases, advanced tumor stage and reduced patient survival time. In vivo and in vitro experiments showed that NAMPT knockdown reduced cell proliferation, migration, invasion and induced apoptosis. Thus, NAMPT could be regarded as a potential prognostic biomarker and therapeutic target for some diseases.
Services at Creative Biolabs
Creative Biolabs offers one-stop services for NAMPT analysis using our advanced protein analysis platform. We offer significant cost saving advantages and record speed in ELISA/WB/IHC analysis. We also have extensive experience and expertise in handling various types of gene level assays. Besides, Creative Biolabs provides a sensitive and robust method to evaluate activators and inhibitors of NAMPT activity using recombinant NAMPT protein.
Our Service Include but not Limited to:
- ELISA: Serum NAMPT levels could be quantified
- Immunohistochemical microarray analysis
- Gene set enrichment analysis
- NAMPT shRNA design and lentivirus construction
- qPCR
- WB Analysis
- Single-nucleotide polymorphism analysis
- NAMPT activity analysis
If you are interested in our services, please feel free to contact us for more information.
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Reference
- Galli, Ubaldina, et al. "Recent advances in NAMPT inhibitors: a novel immunotherapic strategy." Frontiers in pharmacology 11 (2020): 656. Distributed under Open Access license CC BY 4.0, without modification.
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