ERK Signaling Phospho Antibody Array features 227 high-specificity antibodies targeting key nodes in the ERK signaling pathway, optimized for protein phosphorylation profiling in human, mouse, and rat cell and tissue samples. Each kit includes two identical array slides, enabling parallel analysis of two independent samples, such as a control specimen and a treated experimental sample.
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Product Property | |
|---|---|
| Species Reactivity | Human; Mouse; Rat |
| Sample Type | Cell lysate; Tissue lysate |
| Assay Type | Fluorescent Immunoassay |
| Detection Method | Fluorescence Scanning |
| Internal Controls | Beta-actin; GAPDH; Negative controls |
| Principle | Fluorescent immunoassay based on antibody microarray, for quantitative detection of ERK pathway phosphorylation levels. |
| Platform | Fluorescent Antibody Microarray |
| Specificity | Human |
| Cross-Reaction | Mouse; Rat |
| Product Content | 2 array slides per package for two samples (untreated vs. treated) |
| Slide Dimensions | 76 x 25 x 1 mm |
| Application Notes | ELISA based antibody array |
| Core Highlights | Site-specific phosphorylation profiling and screening with 227 antibodies Antibodies covalently immobilized on 3D polymer coated glass slide Fluorescent detection |
| Signaling Pathway | ERK Signaling Pathway |
| Storage | 2-8°C |
Target Information | |
|---|---|
| Analyte | ERK Pathway Phosphoproteins |
| Targets | A-Raf(Tyr301/302), Arrestin-1(Ser412), BAD (Ab-91/128), BAD(Ser112), BAD(Ser134), BAD(Ser136), BAD(Ser155), BAD(Ser91/128), B-Raf(Ser446), B-Raf(Ser601), B-Raf(Thr598), CaMK1-alpha(Thr177), CaMK1-beta, CaMK2(Thr286), CaMK2-beta/gamma/delta(Thr287), CaMK4(Thr196/200), c-Raf(Ser296), c-Raf(Ser43), CREB(Ser121), CREB(Ser129), CREB(Ser133), CREB(Ser142), CREB(Thr100), DYN1(Ser774), Elk1(Ser383), Elk1(Ser389), Elk1(Thr417), ERK3(Ser189), ERK8(Thr175/Tyr177), Fos(Thr232), FosB(Ser27), GRB2(Ser159), GTPase activating protein (Phopspho-Ser387), Histone H3.1(Ser10), IkB-alpha(Ser32/36), IkB-alpha(Tyr42), IKK-alpha(Thr23), IKK-alpha/beta(Ser180/181), IP3KA, IP3KC, IP6K2, IP6K3, MEK1(Ser217), MEK1(Ser221), MEK1(Ser298), MEK1(Thr286), MEK1(Thr291), MEK2(Thr394), MEKKK1, MKK3(Ser189), MKK3/MAP2K3(Thr222), MKK6(Ser207), MKK7/MAP2K7(Ser271), MKK7/MAP2K7(Thr275), Mnk1(Thr385), MSK1(Ser212), MSK1(Ser360), MSK1(Ser376), MSK1(Thr581), MSK2(Thr568), Myc(Ser373), Myc(Ser62), Myc(Thr358), Myc(Thr58), p44/42 MAP Kinase(Thr202), p44/42 MAP Kinase(Thr202/Tyr204), p44/42 MAP Kinase(Tyr204), PAK1(Ser204), PAK1(Thr212), PAK1/2(Ser199), PAK1/2/3(Ser141), PAK1/2/3(Thr423/402/421), PAK2(Ser192), PAK3(Ser154), PI3-kinase p85-alpha(Tyr607), PI3-kinase p85-subunit alpha/gamma(Tyr467/Tyr199), PKA CAT(Thr197), PKC alpha (Tyr657), PKC alpha/beta II(Thr638), PKC beta(Ser661), PKC delta(Ser645), PKC delta(Thr505), PKC epsilon(Ser729), PKC theta(Ser676), PKC theta(Thr538), PKC zeta(Thr410), PKC zeta(Thr560), PKR(Thr446), PKR(Thr451), PLC-beta(Ser1105), PLC-beta3(Ser537), PLCG1(Tyr771), PLCG1(Tyr783), PLCG2(Tyr1217), PLCG2(Tyr753), PP2A-a(Tyr307), Pyk2(Tyr402), Pyk2(Tyr579), Pyk2(Tyr580), Pyk2(Tyr881), Rac1/cdc42(Ser71), Raf1(Ser259), Raf1(Ser338), Raf1(Ser621), Raf1(Tyr341), RapGEF1(Tyr504), RAS (p21 H&K), RASE, RASF4, Ras-GRF1(Ser916), Rho/Rac guanine nucleotide exchange factor 2(Ser885), RSK1/2/3/4(Ser221/227/218/232), Src(Ser75), Src(Tyr418), Src(Tyr529), SRF(Ser77), SRF(Ser99), STAT1(Ser727), STAT1(Tyr701), STAT3(Ser727), STAT3(Tyr705) |
| Target Source | Human; Mouse; Rat |
For Research Use Only.