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General Product Property | |
|---|---|
| Description | Goat Antibody to Human IgG and IgM and IgA, Heavy & Light Chains |
| Host | Goat |
| Species Reactivity | Human |
| Isotype | N/A |
| Clonality | Polyclonal |
| Conjugation | HRP |
| Specificity | Based on Immunoelectrophoresis and or ELISA, the antibody reacts with the heavy chains on Human IgA, IgG and IgM and with light chains common to most human immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins but the antibody may cross-react with immunoglobulins from other species. |
| Format | Lyophilized |
| Purification | Affinity chromatography |
| Purity | >90% by SDS-PAGE |
| Buffer | Lyophilized from 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.6 containing 15 mg/mL BSA (IgG Free and Protease Free). |
| Preservative | None |
| Sterility | All sera are tested for the absence of aerobic and anaerobic bacteria, fungi, yeast and Mycoplasma. |
| Application | IHC; WB; ELISA; Block |
| Application Note | IHC (1:5000-1:100,000); ELISA (1:10,000-1:200,000); WB (1:10,000-1:200,000) |
| Handing advice | Reconstitute to 2 mL distilled water. Centrifuge product if it is not completely clear after standing for 1-2 hours at room temperature. To judge clarity, draw product into a clean pasteur pipette. |
| Storage | Store lyophilized product at 2-8°C. After reconstitution, product is stable for several weeks at 2-8°C as an undiluted liquid. For extended storage, we suggest the addition of an equal volume of glycerol to make a final glycerol concentration of 50% followed by storage at -20°C, with or without aliquotting. |
For Research Use Only.
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