Human DAN antibody pair set for ELISA useCapture Antibody: mouse anti-DAN monoclonal antibodyDetection Antibody: mouse anti-DAN monoclonal antibody conjugated to HRP
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Antigen Detail | |
|---|---|
| Target | DAN |
| Description | The Dan (Differential screening-selected gene aberrative in neuroblastoma, also known as N03) gene was first identified as the putative rat tumor suppressor gene and encodes a protein structurally related to Cerberus and Gremlin in the vertebrates. It is a founding member of the DAN family of secreted proteins, acts as an inhibitor of cell cycle progression, and is closely involved in retinoic acid-induced neuroblastoma differentiation. There are at least five mammalian protein members in the evolutionarily conserved Dan family including DAN, Gremlin/DRM, Cer1 (Cerberus-related), Dante, and PRDC (protein related to DAN and Cerberus), and share the C-terminal cystine-knot motif. As a secreted glycoprotein, DAN is a member of a class of glycoproteins shown to be secreted inhibitors of the transforming growth factor-beta (TGF-beta) and bone morphogenic protein pathways. It binds to BMPs and preventing their interactions with signaling receptor complexes, and accordingly regulates the processes of embryonic development and tissue differentiation. DAN gene product may have an important role in the regulation of the entry of cells into the S phase. Besides, the DAN gene product possesses an ability to revert phenotypes of transformed rat fibroblasts and represents a candidate tumor suppressor gene for neuroblastoma. |
| Species Reactivity | Human |
Capture Antibody | |
|---|---|
| Capture Clonality | Monoclonal |
| Host Species | Rabbit |
| Clone | IV01979 |
| Purification | Affinity Chromatography |
| Buffer | Prior to lyophilization: PBS, pH 7.4 |
| Application | ELISA |
Detection Antibody | |
|---|---|
| Det-Clonality | Monoclonal |
| Host Species | Rabbit |
| Conjugation | HRP |
| Clone | RD10233 |
| Purification | Affinity Chromatography |
| Buffer | Prior to lyophilization: PBS, containing 50 % HRP-Protector |
General Product Property | |
|---|---|
| Product Overview | Human DAN antibody pair set for ELISA use Capture Antibody: mouse anti-DAN monoclonal antibody Detection Antibody: mouse anti-DAN monoclonal antibody conjugated to HRP |
| Specificity | Quantitative determination of Human DAN |
| Range | 46.88-3000 pg/mL |
| Research Area | Suppressor |
| Application | ELISA |
| Application Notes | Optimal dilutions should be determined experimentally by the user. |
| Form | Lyophilized |
| Storage | Avoid repeated freeze/thaw cycles. Store at 2-8°C for short term. Aliquot and store at -20°C for long term storage. |
| Size | 1 Set |
| Sample Type | Serum; Supernatant; Urine; Cell lysate; Plasma; Tissue Homogenates |
| Usage Statement | Available for Research Use Only. Not for use in therapeutic or diagnostic applications. |
Target Overview | |
|---|---|
| Gene Symbol | NBL1 |
| Full Name | Neuroblastoma suppression of tumorigenicity 1 |
| Background | This gene product is the founding member of the evolutionarily conserved CAN (Cerberus and DAN) family of proteins, which contain a domain resembling the CTCK (C-terminal cystine knot-like) motif found in a number of signaling molecules. These proteins are secreted, and act as BMP (bone morphogenetic protein) antagonists by binding to BMPs and preventing them from interacting with their receptors. They may thus play an important role during growth and development. Alternatively spliced transcript variants have been identified for this gene. Read-through transcripts between this locus and the upstream mitochondrial inner membrane organizing system 1 gene (GeneID 440574) have been observed. |
| Alternative Names | Neuroblastoma suppression of tumorigenicity 1; NBL1; No3; NO 3 |
| Gene ID | 4861 |
| Uniprot ID | P41271 |
For Research Use Only.
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