Human DUT Matched Antibody Pair set for ELISA useCapture Antibody: Mouse monoclonal anti-DUTDetection Antibody: Rabbit purified polyclonal anti-DUT
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Antigen Detail | |
|---|---|
| Target | DUT |
| Description | DUTP pyrophosphatase, also known as DUT, is an enzyme which in humans is encoded by the DUT gene on chromosome 15. Diseases associated with DUT include Cysticercosis. |
| Species Reactivity | Human |
Capture Antibody | |
|---|---|
| Capture Clonality | Monoclonal |
| Host Species | Mouse |
| Clone | IV01721 |
| Purification | Affinity chromatography |
| Buffer | PBS, pH 7.4, containing 0.1% Proclin 300 |
| Application | ELISA |
Detection Antibody | |
|---|---|
| Det-Clonality | Polyclonal |
| Host Species | Rabbit |
| Conjugation | Unconjugated |
| Purification | Affinity chromatography |
| Buffer | PBS, pH 7.4 |
General Product Property | |
|---|---|
| Product Overview | Human DUT Matched Antibody Pair set for ELISA use Capture Antibody: Mouse monoclonal anti-DUT Detection Antibody: Rabbit purified polyclonal anti-DUT |
| Specificity | Validated for detecting natural and recombinant human DUT |
| Research Area | Metabolism |
| Application | ELISA |
| Application Notes | Optimal dilutions should be determined experimentally by the user. |
| Form | Lyophilized |
| Storage | Avoid repeated freeze/thaw cycles. Store at 2-8°C for short term. Aliquot and store at -20°C for long term storage. |
| Size | 1 Set |
| Sample Type | Serum; Supernatant; Urine; Cell lysate; Plasma; Tissue Homogenates |
| Usage Statement | Available for Research Use Only. Not for use in therapeutic or diagnostic applications. |
Target Overview | |
|---|---|
| Gene Symbol | DUT |
| Full Name | Deoxyuridine Triphosphatase |
| Background | This gene encodes an essential enzyme of nucleotide metabolism. The encoded protein forms a ubiquitous, homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate. This reaction serves two cellular purposes: providing a precursor (dUMP) for the synthesis of thymine nucleotides needed for DNA replication, and limiting intracellular pools of dUTP. Elevated levels of dUTP lead to increased incorporation of uracil into DNA, which induces extensive excision repair mediated by uracil glycosylase. This repair process, resulting in the removal and reincorporation of dUTP, is self-defeating and leads to DNA fragmentation and cell death. Alternative splicing of this gene leads to different isoforms that localize to either the mitochondrion or nucleus. A related pseudogene is located on chromosome 19. |
| Alternative Names | DUTP Nucleotidohydrolase; DUTP Diphosphatase |
| Gene ID | 1854 |
| Uniprot ID | P33316 |
| HGNC | 3078 |
| Ensembl | ENSG00000128951 |
| OMIM | 601266 |
| Involvement in Disease | Diseases associated with DUT include Cysticercosis. |
| Function | This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA. |
| Related Pathways | Its related pathways are superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis and Metabolism. |
For Research Use Only.
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