Human EPHB3 Matched Antibody Pair set for ELISA useCapture Antibody: Mouse monoclonal anti-EPHB3, IgG1 KappaDetection Antibody: Mouse monoclonal anti-EPHB3, IgG2a Kappa
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Antigen Detail | |
|---|---|
| Target | EPHB3 |
| Description | Ephrin type-B receptor 3 is a protein that in humans is encoded by the EPHB3 gene. EphB2 and EphB3 are involved in the control of thymic epithelial cells (TEC) survival and that the absence of these molecules causes increased apoptotic TEC. EphB2 and EphB3 reverse signaling are critical for the normal development of the projection from the ventral cochlear nucleus to the contralateral medial nucleus of the trapezoid body. |
| Species Reactivity | Human |
Capture Antibody | |
|---|---|
| Capture Clonality | Monoclonal |
| Host Species | Mouse |
| Clone | IV01538 |
| Isotype | IgG1, kappa |
| Purification | Affinity chromatography |
| Buffer | PBS, pH 7.4, containing 0.1% Proclin 300 |
| Application | ELISA |
Detection Antibody | |
|---|---|
| Det-Clonality | Monoclonal |
| Host Species | Mouse |
| Conjugation | Biotin |
| Clone | RD1014 |
| Isotype | IgG2a, kappa |
| Purification | Affinity chromatography |
| Buffer | PBS, pH 7.4 |
General Product Property | |
|---|---|
| Product Overview | Human EPHB3 Matched Antibody Pair set for ELISA use Capture Antibody: Mouse monoclonal anti-EPHB3, IgG1 Kappa Detection Antibody: Mouse monoclonal anti-EPHB3, IgG2a Kappa |
| Specificity | Validated for detecting natural and recombinant human EPHB3 |
| Research Area | Receptor tyrosine kinase |
| Application | ELISA |
| Application Notes | Optimal dilutions should be determined experimentally by the user. |
| Form | Lyophilized |
| Storage | Avoid repeated freeze/thaw cycles. Store at 2-8°C for short term. Aliquot and store at -20°C for long term storage. |
| Size | 1 Set |
| Sample Type | Serum; Supernatant; Urine; Cell lysate; Plasma; Tissue Homogenates |
| Usage Statement | Available for Research Use Only. Not for use in therapeutic or diagnostic applications. |
Target Overview | |
|---|---|
| Gene Symbol | EPHB3 |
| Full Name | EPH Receptor B3 |
| Background | Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into two groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. This gene encodes a receptor for ephrin-B family members. |
| Alternative Names | EK2; Tyro6 |
| Gene ID | 2049 |
| Uniprot ID | P54753 |
| GeneCards | EPHB3 |
| HGNC | 3394 |
| Ensembl | ENSG00000182580 |
| OMIM | 601839 |
| Involvement in Disease | Diseases associated with EPHB3 include Craniofrontonasal Syndrome and Orofacial Cleft. |
| Function | Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Generally has an overlapping and redundant function with EPHB2. Like EPHB2, functions in axon guidance during development regulating for instance the neurons forming the corpus callosum and the anterior commissure, 2 major interhemispheric connections between the temporal lobes of the cerebral cortex. In addition to its role in axon guidance plays also an important redundant role with other ephrin-B receptors in development and maturation of dendritic spines and the formation of excitatory synapses. Controls other aspects of development through regulation of cell migration and positioning. This includes angiogenesis, palate development and thymic epithelium development for instance. Forward and reverse signaling through the EFNB2/EPHB3 complex also regulate migration and adhesion of cells that tubularize the urethra and septate the cloaca. Finally, plays an important role in intestinal epithelium differentiation segregating progenitor from differentiated cells in the crypt. |
| Related Pathways | Its related pathways are Activation of cAMP-Dependent PKA and Actin Nucleation by ARP-WASP Complex. |
For Research Use Only.
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