Human ERCC6L Matched Antibody Pair set for ELISA useCapture Antibody: Rabbit purified polyclonal anti-ERCC6LDetection Antibody: Mouse polyclonal anti-ERCC6L
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Antigen Detail | |
|---|---|
| Target | ERCC6L |
| Description | ERCC6L is a DNA helicase that acts as an essential component of the spindle assembly checkpoint. ERCC6L contributes to the mitotic checkpoint by recruiting MAD2 to kinetochores and monitoring tension on centromeric chromatin. ERCC6L acts as a tension sensor that associates with catenated DNA which is stretched under tension until it is resolved during anaphase. |
| Species Reactivity | Human |
Capture Antibody | |
|---|---|
| Capture Clonality | Polyclonal |
| Host Species | Rabbit |
| Purification | Affinity chromatography |
| Buffer | PBS, pH 7.4, containing 0.1% Proclin 300 |
| Application | ELISA |
Detection Antibody | |
|---|---|
| Det-Clonality | Polyclonal |
| Host Species | Mouse |
| Conjugation | Unconjugated |
| Purification | Affinity chromatography |
| Buffer | PBS, pH 7.4 |
General Product Property | |
|---|---|
| Product Overview | Human ERCC6L Matched Antibody Pair set for ELISA use Capture Antibody: Rabbit purified polyclonal anti-ERCC6L Detection Antibody: Mouse polyclonal anti-ERCC6L |
| Specificity | Validated for detecting natural and recombinant human ERCC6L |
| Research Area | Mitosis |
| Application | ELISA |
| Application Notes | Optimal dilutions should be determined experimentally by the user. |
| Form | Lyophilized |
| Storage | Avoid repeated freeze/thaw cycles. Store at 2-8°C for short term. Aliquot and store at -20°C for long term storage. |
| Size | 1 Set |
| Sample Type | Serum; Supernatant; Urine; Cell lysate; Plasma; Tissue Homogenates |
| Usage Statement | Available for Research Use Only. Not for use in therapeutic or diagnostic applications. |
Target Overview | |
|---|---|
| Gene Symbol | ERCC6L |
| Full Name | ERCC Excision Repair 6 Like, Spindle Assembly Checkpoint Helicase |
| Background | This gene encodes a member of the SWItch/Sucrose Non-Fermentable (SWI/SNF2) family of proteins, and contains a SNF2-like ATPase domain and a PICH family domain. One distinguishing feature of this SWI/SNF protein family member is that during interphase, the protein is excluded from the nucleus, and only associates with chromatin after the nuclear envelope has broken down. This protein is a DNA translocase that is thought to bind double-stranded DNA that is exposed to stretching forces, such as those exerted by the mitotic spindle. This protein associates with ribosomal DNA and ultra-fine DNA bridges (UFBs), fine structures that connect sister chromatids during anaphase at some sites such as fragile sites, telomeres and centromeres. This gene is required for the faithful segregation of sister chromatids during mitosis, and the ATPase activity of this protein required for the resolution of UFBs before cytokinesis. |
| Alternative Names | ATP-Dependent Helicase ERCC6-Like; Plk1-Interacting Checkpoint Helicase |
| Gene ID | 54821 |
| Uniprot ID | Q2NKX8 |
| HGNC | 20794 |
| Ensembl | ENSG00000186871 |
| OMIM | 300687 |
| Involvement in Disease | Diseases associated with ERCC6L include Cavernous Hemangioma. |
| Function | DNA helicase that acts as an essential component of the spindle assembly checkpoint. Contributes to the mitotic checkpoint by recruiting MAD2 to kinetochores and monitoring tension on centromeric chromatin (PubMed:17218258). Acts as a tension sensor that associates with catenated DNA which is stretched under tension until it is resolved during anaphase (PubMed:17218258, PubMed:23973328). Functions as ATP-dependent DNA translocase (PubMed:23973328, PubMed:28977671). Can promote Holliday junction branch migration (in vitro) (PubMed:23973328). |
| Related Pathways | Its related pathways are Signaling by GPCR and Mitotic Prometaphase. |
For Research Use Only.
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