Insulin Receptor Phospho Antibody Array features 219 high-specificity antibodies targeting key insulin receptor-related proteins in insulin signaling pathways, optimized for protein phosphorylation profiling in human, mouse, and rat cell and tissue samples. Each kit includes two identical array slides, enabling parallel analysis of two independent samples, such as a control specimen and a treated experimental sample.
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Product Property | |
|---|---|
| Species Reactivity | Human; Mouse; Rat |
| Sample Type | Cell lysate; Tissue lysate |
| Assay Type | Fluorescent Immunoassay |
| Detection Method | Fluorescence Scanning |
| Internal Controls | Beta-actin; GAPDH; Negative controls |
| Principle | Fluorescent immunoassay based on antibody microarray, for quantitative detection of insulin receptor pathway phosphorylation levels. |
| Platform | Fluorescent Antibody Microarray |
| Specificity | Human |
| Cross-Reaction | Mouse; Rat |
| Product Content | 2 array slides per package for two samples (untreated vs. treated) |
| Slide Dimensions | 76 x 25 x 1 mm |
| Application Notes | ELISA based antibody array |
| Core Highlights | Site-specific phosphorylation profiling and screening Suitable samples include: cell lysates; frozen or FFPE tissue lysates Glass based array with high specificity and low background Antibodies covalently attached to 3D polymer coated glass surface Sensitive fluorescent detection |
| Signaling Pathway | Insulin Receptor Signaling Pathway |
| Storage | 2-8°C |
Target Information | |
|---|---|
| Analyte | Insulin Receptor Pathway Phosphoproteins |
| Targets | 4E-BP1 (Ser65), 4E-BP1 (Thr36), 4E-BP1 (Thr45), 4E-BP1 (Thr70), AKT (Ser473), AKT (Thr308), AKT (Tyr326), AKT1 (Ser124), AKT1 (Ser246), AKT1 (Thr450), AKT1 (Thr72), AKT1 (Tyr474), AKT1S1 (Thr246), AKT2 (Ser474), AMPK1 (Thr174), AMPK1/AMPK2 (Ser485/491), AMPKbeta1 (Ser182), ATP-Citrate Lyase (Ser454), BAD (Ser112), BAD (Ser134), BAD (Ser136), BAD (Ser155), BAD (Ser91/128), Caveolin-1 (Tyr14), CBL (Tyr700), CBL (Tyr774), c-Raf (Ser296), c-Raf (Ser43), CrkII (Tyr221), CrkL (Tyr207), eIF2a (Ser51), eIF4B (Ser422), eIF4E (Ser209), eIF4G (Ser1108), ERK3 (Ser189), ERK8 (Thr175/Tyr177), FKHR (Ser256), FKHR (Ser319), FKHRL1 (Ser253), FOXO1/3/4-PAN (Thr24/32), FOXO1A (Ser329), FOXO1A/3A (Ser322/325), Gab1 (Tyr627), Gab1 (Tyr659), GRB2 (Ser159), GRB10 (Tyr67), GSK3a (Ser21), GSK3a/b (Tyr216/279), GSK3b (Ser9), HSL (Ser552/563), HSL (Ser554), IKKa (Thr23), IKKa/b (Ser180/181), IKKb (Tyr188), IKKb (Tyr199), IKKg (Ser31), IKKg (Ser85), IR (Tyr1355), IR (Tyr1361), IRS-1 (Ser1101), IRS-1 (Ser307), IRS-1 (Ser312), IRS-1 (Ser323), IRS-1 (Ser612), IRS-1 (Ser636), IRS-1 (Ser639), IRS-1 (Ser794), LKB1 (Ser428), LKB1 (Thr189), MEK1 (Ser217), MEK1 (Ser221), MEK1 (Ser298), MEK1 (Thr286), MEK1 (Thr291), MEK2 (Thr394), mTOR (Ser2448), mTOR (Ser2481), mTOR (Thr2446), p44/42MAPK (Thr202), ERK1-p44/42MAPK (Tyr204), p70S6K beta (Ser423), p70S6K (Ser371), p70S6K (Ser411), p70S6K (Ser418), p70S6K (Ser424), p70S6K (Thr229), p70S6K (Thr389), p70S6K (Thr421), PDK1 (Ser241), PI3K-p85-a (Tyr607), PI3K-p85-a/g (Tyr467/Tyr199), PKA CAT (Thr197), PKC pan activation site, PKC theta (Ser676), PKC theta (Thr538), PKC zeta (Thr410), PKC zeta (Thr560), PP1a (Thr320), PP2A-a (Tyr307), PTEN (Ser370), PTEN (Ser380), PTEN (Ser380/Thr382/Thr383), PTPRA (Tyr798), RASE, RASF4, Ras-GRF1 (Ser916), Shc (Tyr349), Shc (Tyr427), SHP-2 (Tyr542), SHP-2 (Tyr580), TNFR1, TNFR2, Tuberin (Ser939), Tuberin (Thr1462) |
| Target Source | Human; Mouse; Rat |
For Research Use Only.