Mouse IFNγ antibody pair set for ELISPOT useCapture Antibody: Rat Monoclonal IgGDetection Antibody: Rat Monoclonal IgG
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Antigen Detail | |
|---|---|
| Target | IFNγ |
| Description | Interferon gamma (IFNγ) is a dimerized soluble cytokine that is the only member of the type II class of interferons. The existence of this interferon, which early in its history was known as immune interferon, was described by E. F. Wheelock as a product of human leukocytes stimulated with phytohemagglutinin, and by others as a product of antigen-stimulated lymphocytes. It was also shown to be produced in human lymphocytes or tuberculin-sensitized mouse peritoneal lymphocytes challenged with PPD; the resulting supernatants were shown to inhibit growth of vesicular stomatitis virus. Those reports also contained the basic observation underlying the now widely employed interferon gamma release assay used to test for tuberculosis. In humans, the IFNγ protein is encoded by the IFNG gene. |
| Species Reactivity | Mouse |
Capture Antibody | |
|---|---|
| Capture Clonality | Monoclonal |
| Host Species | Rat |
| Clone | IV01921 |
| Isotype | IgG |
| Purification | Affinity Chromatography |
| Buffer | Aqueous solution containing 0.09% sodium azide |
| Application | ELISA |
Detection Antibody | |
|---|---|
| Det-Clonality | Monoclonal |
| Host Species | Rat |
| Conjugation | Biotin |
| Clone | RD1049 |
| Isotype | IgG |
| Purification | Affinity Chromatography |
| Buffer | Aqueous solution containing 0.09% sodium azide |
General Product Property | |
|---|---|
| Product Overview | Mouse IFNγ antibody pair set for ELISPOT use Capture Antibody: Rat Monoclonal IgG Detection Antibody: Rat Monoclonal IgG |
| Specificity | Both the capture and the detection antibodies in this set react with mouse IFNγ protein. |
| Research Area | Inflammation |
| Application | ELISPOT |
| Application Notes | Optimal dilutions should be determined experimentally by the user. |
| Form | Liquid |
| Storage | Avoid repeated freeze/thaw cycles. Store at 2-8°C for short term. Aliquot and store at -20°C for long term storage. |
| Size | 1 Set |
| Sample Type | Plasma; Serum; Cell Culture Supernatant |
| Usage Statement | Available for Research Use Only. Not for use in therapeutic or diagnostic applications. |
Target Overview | |
|---|---|
| Gene Symbol | IFNG |
| Full Name | IFN gamma |
| Background | Interferon gamma (IFNγ) is a dimerized soluble cytokine that is the only member of the type II class of interferons.[1] The existence of this interferon, which early in its history was known as immune interferon, was described by E. F. Wheelock as a product of human leukocytes stimulated with phytohemagglutinin, and by others as a product of antigen-stimulated lymphocytes.[2] It was also shown to be produced in human lymphocytes.[3] or tuberculin-sensitized mouse peritoneal lymphocytes[4] challenged with PPD; the resulting supernatants were shown to inhibit growth of vesicular stomatitis virus. Those reports also contained the basic observation underlying the now widely employed interferon gamma release assay used to test for tuberculosis. In humans, the IFNγ protein is encoded by the IFNG gene. |
| Alternative Names | IFNG; IFN gamma |
| Gene ID | 3459(Human) |
| Uniprot ID | P15260(Human) |
| GeneCards | IFNG |
| HGNC | HGNC(5438) |
| Ensembl | Ensembl(ENSG00000111537) |
| OMIM | OMIM(147570) |
| Involvement in Disease | Diseases associated with IFNG include Hepatitis C Virus and Tuberous Sclerosis 2. |
| Function | Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. |
| Related Pathways | Its related pathways are Folate Metabolism and Tuberculosis. |
For Research Use Only.
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