Mouse IL-10 antibody pair set for ELISA useCapture Antibody: Rat Monoclonal IgMDetection Antibody: Rat Monoclonal IgG1
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Antigen Detail | |
|---|---|
| Target | IL-10 |
| Description | Interleukin 10 (IL-10), also known as human cytokine synthesis inhibitory factor (CSIF), is an anti-inflammatory cytokine. In humans, interleukin 10 is encoded by the IL10 gene. IL-10 signals through a receptor complex consisting of two IL-10 receptor-1 and two IL-10 receptor-2 proteins. Consequently, the functional receptor consists of four IL-10 receptor molecules. IL-10 binding induces STAT3 signalling via the phosphorylation of the cytoplasmic tails of IL-10 receptor 1 + IL-10 receptor 2 by JAK1 and Tyk2 respectively. |
| Species Reactivity | Mouse |
Capture Antibody | |
|---|---|
| Capture Clonality | Monoclonal |
| Host Species | Rat |
| Production | Cell culture |
| Clone | IV01374 |
| Isotype | IgM |
| Purification | Ammonium sulphate precipitation and DEAE ion-exchange chromatography |
| Buffer | Prior to lyophilization: 0.25 ml PBS containing 125 mM trehalose |
| Application | ELISA |
Detection Antibody | |
|---|---|
| Det-Clonality | Monoclonal |
| Host Species | Rat |
| Production | Cell culture |
| Conjugation | Biotin |
| Clone | RD10241 |
| Isotype | IgG1 |
| Purification | Ammonium sulphate precipitation, protein A- and DEAE-chromatography. |
| Buffer | Prior to lyophilization: 0.5 ml PBS, containing 1% BSA and 125 mM trehalose |
General Product Property | |
|---|---|
| Product Overview | Mouse IL-10 antibody pair set for ELISA use Capture Antibody: Rat Monoclonal IgM Detection Antibody: Rat Monoclonal IgG1 |
| Specificity | Validated for detecting natural and recombinant mouse IL-10. |
| Sensitivity | 32 pg/ml |
| Research Area | Immunology; Inflammation |
| Application | ELISA |
| Application Notes | Optimal dilutions should be determined experimentally by the user. |
| Form | Lyophilized |
| Storage | Avoid repeated freeze/thaw cycles. Store at 2-8°C for short term. Aliquot and store at -20°C for long term storage. |
| Size | 1 Set |
| Sample Type | Serum; Supernatant; Urine; Cell lysate; Plasma; Tissue Homogenates |
| Usage Statement | Available for Research Use Only. Not for use in therapeutic or diagnostic applications. |
Target Overview | |
|---|---|
| Gene Symbol | IL10 |
| Full Name | Interleukin 10 |
| Background | The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis. |
| Alternative Names | IL10; CSIF; GVHDS; IL-10; IL10A; TGIF; interleukin 10 |
| Gene ID | 3586 |
| Uniprot ID | P22301 |
| GeneCards | IL10 |
| HGNC | 5962 |
| Ensembl | ENSG00000136634 |
| OMIM | 124092 |
| Involvement in Disease | Diseases associated with IL10 include Graft-Versus-Host Disease and Human Immunodeficiency Virus Type 1. |
| Function | Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3 (PubMed:16982608). In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators (PubMed:18025162). Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha (PubMed:1940799, PubMed:7512027, PubMed:11564774). Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation (PubMed:8144879). In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling (By similarity). |
| Related Pathways | Its related pathways are Tuberculosis and PEDF Induced Signaling. |
For Research Use Only.
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