Mouse Lactadherin Matched Antibody Pair Set [ABP-P-668] (CAT#: ABP-P-668)

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The antibody pair set can be used to detect Mouse Lactadherin by sandwich ELISA. This antigen affinity purified capture antibody, combined with biotinylated detection antibody, allows for the detection of recombinant Lactadherin.

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Lot Number

Antigen Detail
TargetLactadherin
DescriptionLactadherin, a novel adhesion molecule, also known as MFG-E8, is a widely distributed glycoprotein (~50 kD) that has been found in macrophages, dendritic cells, vascular smooth muscle cells, astrocytes, microglia, breast carcinioma cells, and also in breast milk and around blood vessels. Lactadherin contains an N-terminal EGF-like motif with an inserted RGD cell adhesion sequence; it also contains a C-terminal sequence sharing homology with the discoidin family of lectin domains, including the phospholipid-binding domains of blood clotting factors V and VIII.
Species ReactivityMouse

Capture Antibody
Capture ClonalityPolyclonal
Host SpeciesGoat
Immunogen293E cells expressed recombinant mouse Lactadherin
IsotypeIgG
PurificationAffinity Chromatography
BufferPBS, pH 7.4, containing 0.1% Proclin 300
ApplicationELISA

Detection Antibody
Det-ClonalityPolyclonal
Host SpeciesGoat
Immunogen293E cells expressed recombinant mouse Lactadherin
ConjugationBiotin
IsotypeIgG
PurificationAffinity Chromatography
ApplicationPBS, pH 7.2, containing 0.09% sodium azide.

General Product Property
Product OverviewThe antibody pair set can be used to detect Mouse Lactadherin by sandwich ELISA. This antigen affinity purified capture antibody, combined with biotinylated detection antibody, allows for the detection of recombinant Lactadherin.
Research AreaImmunology
ApplicationELISA
Application NotesOptimal dilutions should be determined experimentally by the user.
FormLiquid
StorageAvoid repeated freeze/thaw cycles. Store at 2-8°C for short term. Aliquot and store at -20°C for long term storage.
Size1 Set
Sample TypeSerum; Plasma; Cell Culture Supernatant
Usage StatementEach lot of this antibody is quality control tested by ELISA assay. For ELISA Capture applications, a concentration range of 0.5 - 4 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of LIF recombinant protein ranging from 1200 to 18.75 pg/mL is recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Target Overview
Gene SymbolLactadherin
BackgroundLactadherin, a novel adhesion molecule, also known as MFG-E8, is a widely distributed glycoprotein (~50 kD) that has been found in macrophages, dendritic cells, vascular smooth muscle cells, astrocytes, microglia, breast carcinioma cells, and also in breast milk and around blood vessels. Lactadherin contains an N-terminal EGF-like motif with an inserted RGD cell adhesion sequence; it also contains a C-terminal sequence sharing homology with the discoidin family of lectin domains, including the phospholipid-binding domains of blood clotting factors V and VIII.
Alternative NamesLactadherin; Milk fat globule-EGF factor 8; MFG-E8; MFGE-8; MFGE8 MFGM; Sperm surface protein SP47; MP47 PAS-6/7; bovine-associated mucoprotein; BA-46; P47
Gene ID17304(Mouse)
Uniprot IDP21956(Mouse)

For Research Use Only.

Application
AgonistAgonist
AntagonistAntagonist
ApopApoptosis Assay
BlockBlocking
BIBioimaging
ChIPChromatin Immunoprecipitation
Co-IPCo-Immunoprecipitation
CytCytotoxicity Assay
CellActCell Activiation
CellDiffCell Differentiation
DBDot Blot
ECLElectrochemiluminescence
ELISAEnzyme-Linked Immunosorbent Assay
Indirect ELISAIndirect ELISA
Sandwich ELISASandwich ELISA
Competitive ELISACompetitive ELISA
ELISPOTEnzyme-linked Immunosorbent Spot
FuncSFunctional Studies
FACSFluorescence-activated cell sorting
FCFlow Cytometry
FIAFluorescence Immunoassay
FRETFluorescence Resonance Energy Transfer Microscopy
GSGel Shift
HAHemagglutination Assay
HPLCHigh Performance Liquid Chromatography
IAImmunoassay
IBImmunoblotting
ICCImmunocytochemistry
IEMImmuno-Electron Microscopy
IFImmunofluorescence
IHCImmunohistochemistry
IHC-FrImmunohistochemistry-Frozen
IHC-PImmunohistochemistry-Paraffin
IPImmunoprecipitation
IRMAImmunoradiometric Assay
ISHIn Situ Hybridization
InhibitInhibition Assay
Live-cell imagingLive-cell Imaging Assay
MeDIPMethylated DNA Immunoprecipitation
MAMicroagglutination Assay
MSMass Spectrometry
NeutNeutralization Assay
PepArrPeptide Array
PPProtein Purification
PGProteogenomics
RIRadial Immunodiffusion
RIARadioimmunoassay
RIPRadioimmunoprecipitation
StimStimulation Assay
TCAT-Cell Activation Assay
TRFTime-Resolved Fluorescence
TCTissue Culture
TBTurbidimetry
PBAProtein Binding Assay
PLAProximity Ligation Assay
SDS-PAGESodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis
SPRSurface Plasmon Resonance
WBWestern Blot
CLIAChemiluminescent Immunoassay
CLEIAChemiluminescent Enzyme Immunoassay
ECLIAElectrochemiluminescence Immunoassay
TRFIATime-resolved Fluorescence Immunoassay
LIALaser Immunoassay
MLEIAMicroparticle Luminescence Enzyme Immunoassay
LETIALatex Particle-Enhanced Turbidimetric Immunoassay
LFIALateral Flow Immunoassay
GICAColloidal Gold immunochomatography Assay
ICTImmune Chromatographic Test
LTIALatex Turbidimetric Immunoassay
CMIAChemiluminescence microparticle immuno assay
IACInnuno-Affinity Chromatography
TIATurbidimetric Immunoassay
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