Porcine IFNγ antibody pair set for ELISA useCapture Antibody: Mouse Monoclonal IgGDetection Antibody: Mouse MonoclonalIgG
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Antigen Detail | |
|---|---|
| Target | IFNγ |
| Description | IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway. IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma. IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia. |
| Species Reactivity | Porcine |
Capture Antibody | |
|---|---|
| Capture Clonality | Monoclonal |
| Host Species | Mouse |
| Clone | IV01969 |
| Isotype | IgG |
| Purification | Affinity Chromatography |
| Buffer | PBS, pH 7.4, containing 0.1% Proclin 300 |
| Application | ELISA |
Detection Antibody | |
|---|---|
| Det-Clonality | Monoclonal |
| Host Species | Mouse |
| Conjugation | Biotin |
| Clone | RD1030 |
| Isotype | IgG |
| Purification | Affinity Chromatography |
| Buffer | PBS, pH 7.4, containing 0.03% Proclin 300 |
General Product Property | |
|---|---|
| Product Overview | Porcine IFNγ antibody pair set for ELISA use Capture Antibody: Mouse Monoclonal IgG Detection Antibody: Mouse MonoclonalIgG |
| Specificity | Both the capture and the detection antibodies in this set react with Porcine IFNγ. |
| Sensitivity | 15.6 pg/mL |
| Range | 15.6-1000 pg/mL |
| Research Area | Immunology |
| Application | ELISA |
| Application Notes | Optimal dilutions should be determined experimentally by the user. |
| Form | Liquid |
| Storage | Avoid repeated freeze/thaw cycles. Store at 2-8°C for short term. Aliquot and store at -20°C for long term storage. |
| Size | 1 Set |
| Sample Type | Plasma; Serum; Cell Culture Supernatant |
| Usage Statement | Available for Research Use Only. Not for use in therapeutic or diagnostic applications. |
Target Overview | |
|---|---|
| Gene Symbol | IFNG |
| Full Name | IFN gamma |
| Background | nterferon gamma (IFNγ) is a dimerized soluble cytokine that is the only member of the type II class of interferons.[1] The existence of this interferon, which early in its history was known as immune interferon, was described by E. F. Wheelock as a product of human leukocytes stimulated with phytohemagglutinin, and by others as a product of antigen-stimulated lymphocytes.[2] It was also shown to be produced in human lymphocytes.[3] or tuberculin-sensitized mouse peritoneal lymphocytes[4] challenged with PPD; the resulting supernatants were shown to inhibit growth of vesicular stomatitis virus. Those reports also contained the basic observation underlying the now widely employed interferon gamma release assay used to test for tuberculosis. In humans, the IFNγ protein is encoded by the IFNG gene. |
| Alternative Names | IFNG; IFN gamma |
| Gene ID | 3459(Human) |
| Uniprot ID | P15260(Human) |
| GeneCards | IFNG |
| HGNC | HGNC(5438) |
| Ensembl | Ensembl(ENSG00000111537) |
| OMIM | OMIM(147570) |
| Involvement in Disease | Diseases associated with IFNG include Hepatitis C Virus and Tuberous Sclerosis 2. |
| Function | Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. |
| Related Pathways | Its related pathways are Folate Metabolism and Tuberculosis. |
For Research Use Only.
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