T-Cell Receptor Pathway Phospho Antibody Array features 213 high-specificity antibodies targeting key molecules related to T-cell receptor signaling, optimized for protein phosphorylation profiling in human, mouse, and rat cell and tissue samples. Each kit includes two identical array slides, enabling parallel analysis of two independent samples, such as a control specimen and a treated experimental sample.
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Product Property | |
|---|---|
| Species Reactivity | Human; Mouse; Rat |
| Sample Type | Cell lysate; Tissue lysate |
| Assay Type | Fluorescent Immunoassay |
| Detection Method | Fluorescence Scanning |
| Internal Controls | Beta-actin; GAPDH; Negative controls |
| Principle | Fluorescent immunoassay based on antibody microarray, for quantitative detection of T-cell receptor pathway phosphorylation levels. |
| Platform | Fluorescent Antibody Microarray |
| Specificity | Human |
| Cross-Reaction | Mouse; Rat |
| Slide Dimensions | 76 x 25 x 1 mm |
| Application Notes | ELISA based antibody array |
| Core Highlights | Site-specific phosphorylation profiling and screening with 213 antibodies Antibodies covalently immobilized on 3D polymer coated glass slide 6 replicates/antibody Fluorescent detection |
| Signaling Pathway | T-Cell Receptor Signaling Pathway |
| Storage | 2-8°C |
Target Information | |
|---|---|
| Analyte | T-Cell Receptor Pathway Phosphoproteins |
| Targets | AKT(Ser473), AKT(Thr308), AKT(Tyr326), Calmodulin(Thr79/Ser81), CD127, CD134 (OX40), CD137, CD152, CD154, CD197 (CCR7), CD2, CD25, CD27, CD272, CD278 (ICOS), CD279, CD28, CD28(Tyr218), CD3, CD39, CD3Z(Tyr142), CD4, CD4(Ser433), CD45(Ser1007), CD45RA, CD45RO, CD5, CD62L, CD69, CD7, CD73, CD8, c-Jun(Ser243), c-Jun(Ser63), c-Jun(Ser73), c-Jun(Thr239), c-Jun(Thr91), c-Jun(Thr93), c-Jun(Tyr170), ERK1(Thr202), ERK1(Tyr204), ERK1/2, ERK3(Ser189), ERK8(Thr175/Tyr177), Fos(Ser362), Fos(Thr232), FosB(Ser27), GAD1/GAD2, GAD67/GAD1, GADD153, GRB2(Ser159), IkB-alpha(Ser32/36), IkB-alpha(Tyr305), IkB-alpha(Tyr42), IkB-beta(Thr19), IkB-epsilon(Ser22), IKK alpha(Thr23), IKK alpha/beta(Ser180/181), IKK beta(Tyr188), IKK beta(Tyr199), IKK gamma(Ser31), IKK gamma(Ser85), IP3KA, IP3KC, JNK1/2/3(Thr183/Tyr185), LAT(Tyr171), LAT(Tyr191), LAT3, LCK(Ser59), LCK(Tyr192), LCK(Tyr393), LCK(Tyr504), MEK1(Ser217), MEK1(Ser221), MEK1(Ser298), MEK1(Thr286), MEK1(Thr291), MEK2(Thr394), MEKKK1, MEKKK4, MKK3(Ser189), MKK3/MAP2K3(Thr222), MKK6(Ser207), MKK7/MAP2K7(Ser271), MKK7/MAP2K7(Thr275), NFAT3, NFAT4(Ser165), NFkB-p100/p52(Ser865), NFkB-p100/p52(Ser869), NFkB-p105(Ser927), NFkB-p105/p50(Ser337), NFkB-p105/p50(Ser932), NFkB-p65(Ser536), NFkB-p65(Thr254), p38 MAPK(Thr180), p38 MAPK(Tyr182), p38 MAPK(Tyr322), Perforin, PI3K-p85-a(Tyr607), PI3K-p85-a/g(Tyr467/199), PKC pan activation sites, PKC theta(Ser676), PKC theta(Thr538), Rac1/cdc42(Ser71), Raf1(Ser259), Raf1(Ser338), Raf1(Ser621), Raf1(Tyr341), Ras-GRF1(Ser916), Rel(Ser503), RelB(Ser552), SLP-76(Tyr128), VAV(Tyr174), VAV2(Tyr142), WASP(Tyr290), ZAP-70(Tyr292), ZAP-70(Tyr315), Zap-70(Tyr319), Zap-70(Tyr493) |
| Target Source | Human; Mouse; Rat |
For Research Use Only.