VEGF Phospho Antibody Array features 185 high-specificity antibodies targeting key nodes of the VEGF signaling pathway, optimized for protein phosphorylation profiling in human, mouse, and rat cell and tissue samples. Each kit includes two identical array slides, enabling parallel analysis of two independent samples, such as a control specimen and a treated experimental sample.
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Product Property | |
|---|---|
| Species Reactivity | Human; Mouse; Rat |
| Sample Type | Cell lysate; Tissue lysate |
| Assay Type | Fluorescent Immunoassay |
| Detection Method | Fluorescence Scanning |
| Internal Controls | Beta-actin; GAPDH; Negative controls |
| Principle | Fluorescent immunoassay based on antibody microarray, for quantitative detection of VEGF pathway phosphorylation levels. |
| Platform | Fluorescent Antibody Microarray |
| Specificity | Human |
| Cross-Reaction | Mouse; Rat |
| Slide Dimensions | 76 x 25 x 1 mm |
| Application Notes | ELISA based antibody array |
| Core Highlights | Site-specific phosphorylation profiling and screening with 185 antibodies Antibodies covalently immobilized on 3D polymer coated glass slide Fluorescent detection |
| Signaling Pathway | VEGF Signaling Pathway |
| Storage | 2-8°C |
Target Information | |
|---|---|
| Analyte | VEGF Pathway Phosphoproteins |
| Targets | AKT (Ser473), AKT (Thr308), AKT (Tyr326), AKT1 (Thr450), AKT1 (Thr72), AKT2 (Ser474), BAD (Ser112), BAD (Ser134), BAD (Ser136), BAD (Ser155), BAD (Ser91/128), Casp9 (Ser144), Casp9 (Ser196), Casp9 (Tyr153), c-PLA2 (Ser505), Elk1 (Ser383), Elk1 (Ser389), Elk1 (Thr417), eNOS (Ser1177), eNOS (Ser615), eNOS (Thr495), ERK1/2, ERK3 (Ser189), ERK8 (Thr175/Tyr177), FAK (Ser910), FAK (Tyr397), FAK (Tyr407), FAK (Tyr576), FAK (Tyr861), FAK (Tyr925), FKHR (Ser256), FKHR (Ser319), GRB2 (Ser159), GTPase activating protein (Phopspho-Ser387), HSP27 (Ser15), HSP27 (Ser78), HSP27 (Ser82), HSP90 co-chaperone Cdc37 (Ser13), HSP90A, HSP90B (Ser254), IP3KA, IP3KC, MAPKAPK2 (Ser272), MAPKAPK2 (Thr222), MAPKAPK2 (Thr334), MEK1 (Ser217), MEK1 (Ser221), MEK1 (Ser298), MEK1 (Thr286), MEK1 (Thr291), MEK2 (Thr394), MKK3 (Ser189), MKK3 (Thr222), MKK6 (Ser207), P38 MAPK (Thr180), P38 MAPK (Tyr182), P38 MAPK (Tyr322), p44/42 MAPK (Thr202), p44/42 MAPK (Tyr204), Paxillin (Tyr118), Paxillin (Tyr31), PI3K-p85-a (Tyr607), PI3K-p85-a/g (Tyr467/Tyr199), PKC-a (Tyr657), PKC a/b II (Thr638), PKC-b (Ser661), PKC delta (Ser645), PKC delta (Thr505), PKC epsilon (Ser729), PKC theta (Ser676), PKC theta (Thr538), PKC zeta (Ab-410), PKC zeta (Ab-560), PKC zeta (Thr410), PKC zeta (Thr560), PLC-g1 (Tyr1253), PLC-g1 (Tyr771), PLC-g1 (Tyr783), PLC-g2 (Tyr1217), PLC-g2 (Tyr753), Raf1 (Phospho-Ser621), Raf1 (Ser259), Raf1 (Ser338), Raf1 (Tyr341), RapGEF1 (Tyr504), Ras-GRF1 (Ser916), Shc (Tyr349), Shc (Tyr427), Src (Ser75), Src (Tyr216), Src (Tyr418), Src (Tyr529), VEGFR1 (Tyr1333), VEGFR2 (Tyr1054), VEGFR2 (Tyr1059), VEGFR2 (Tyr1175), VEGFR2 (Tyr1214), VEGFR2 (Tyr951) |
| Target Source | Human; Mouse; Rat |
For Research Use Only.