Wnt Signaling Phospho Antibody Array features 227 high-specificity antibodies targeting key functional molecules in the Wnt/β-Catenin pathway, optimized for protein phosphorylation profiling in human, mouse, and rat cell and tissue samples. Each kit includes two identical array slides, enabling parallel analysis of two independent samples, such as a control specimen and a treated experimental sample.
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Product Property | |
|---|---|
| Species Reactivity | Human; Mouse; Rat |
| Sample Type | Cell lysate; Tissue lysate |
| Assay Type | Fluorescent Immunoassay |
| Detection Method | Fluorescence Scanning |
| Internal Controls | Beta-actin; GAPDH; Negative controls |
| Principle | Fluorescent immunoassay based on antibody microarray, for quantitative detection of Wnt pathway phosphorylation levels. |
| Platform | Fluorescent Antibody Microarray |
| Specificity | Human |
| Cross-Reaction | Mouse; Rat |
| Slide Dimensions | 76 x 25 x 1 mm |
| Application Notes | ELISA based antibody array |
| Core Highlights | Site-specific phosphorylation profiling and screening with 227 antibodies Antibodies covalently immobilized on 3D polymer coated glass slide Fluorescent detection |
| Signaling Pathway | Wnt Signaling Pathway |
| Storage | 2-8°C |
Target Information | |
|---|---|
| Analyte | Wnt Pathway Phosphoproteins |
| Targets | AKT (Ser124), AKT (Ser246), AKT (Ser473), AKT (Thr308), AKT (Thr450), AKT (Thr72), AKT (Tyr326), AKT (Tyr474), AKT1 S1 (Thr246), AKT1/2/3 (Tyr315/316/312), AKT2 (Ser474), APC (Ser2054), APC1 (Ser355), APC1 (Ser688), CaMK2a (Thr286), CaMK2a/b/d (Thr305), CaMK2b/g/d (Thr287), Catenin a1 (Ser641), Catenin beta (Ser33), Catenin beta (Ser37), Catenin beta (Thr41/Ser45), Catenin beta (Tyr489), Catenin beta (Tyr654), Catenin delta1 (Tyr228), CKI-a (Tyr294), CKI-a (Tyr321), CKI-g 1/2/3 (Tyr263), CKII-a (Tyr255), CKII-b (Ser209), ERK3 (Ser189), ERK8 (Thr175/Tyr177), GPR120, GPR132, GPR150, GPR151, GPR152, GPR153, GPR160, GPR171, GPR173, GPR174, GPR175, GPR18, GSK3a (Ser21), GSK3a/b (Tyr216/279), GSK3b (Ser9), JNK1/2/3 (Thr183/Tyr185), MAP3K1 (Thr1402), MAP3K10, MAP3K11 (Ser674), MAP3K11 (Thr277/Ser281), MAP3K15, MAP3K3, MAP3K4, MAP3K6, MAP3K7 (Ser439), MAP3K71 (Thr187), MAP3K8 (Ser400), MAP3K9, MAP3KL4, MEK1 (Ser217), MEK1 (Ser221), MEK1 (Ser298), MEK1 (Thr286), MEK1 (Thr291), MEK2 (Thr394), MEKKK1, MEKKK4, mTOR (Ser2448), mTOR (Ser2481), mTOR (Thr2446), NFAT1 (Ser326), NFAT3 (Ser168/Ser170), NFAT3 (Ser676), NFAT4 (Ser165), NFAT5 (Ser1197), P38 MAPK (Thr179/Tyr181), P38 MAPK (Thr180), P38 MAPK (Tyr182), P38 MAPK (Tyr322), P44/42 MAPK, PDE4D (Ser190/53), PI3K-p85-a (Tyr607), PI3K-p85-a/g (Tyr467/Tyr199), PKA-a/b CAT (Thr197), PKA-R2-b (Ser113), PKC-a (Thr638), PKC-a (Tyr657), PKC-b (Ser661), PKC delta (Ser645), PKC delta (Thr505), PKC delta (Tyr313), PKC delta (Tyr52), PKC delta (Tyr64), PKC epsilon (Ser729), PKC pan (Thr497), PKC theta (Ser676), PKC theta (Ser695), PKC theta (Thr538), PKC zeta (Thr410), PKC zeta (Thr560), PKG2 (Ser126), PLC beta 3 (Ser1105), PLC beta 3 (Ser537), PLCG1 (Tyr1253), PLCG1 (Tyr771), PLCG1 (Tyr783), PLCG2 (Tyr1217), PLCG2 (Tyr753), PLCG2 (Tyr759), Rac1/cdc42 (Ser71), Rho/Rac GEF2 (Ser885), RhoA (Ser188), SAPK/JNK (Thr183), SAPK/JNK (Tyr185), Src (Ser75), Src (Tyr216), Src (Tyr418), Src (Tyr529), TAK1 (Thr184), Ubiquitin, VE-Cadherin (Tyr731), WNT1, WNT5A |
| Target Source | Human; Mouse; Rat |
For Research Use Only.