What types of samples can be used for P2RX4 analysis?

We accept a wide range of biological samples, including cell lines, primary cells, and tissues from various species. We can also work with purified protein or gene constructs. Please contact our team to discuss your specific sample type and ensure it is compatible with our assays.

How does your P2RX4 analysis compare to other providers?

Our service stands out due to our unique combination of deep expertise in purinergic signaling and advanced technology platforms. Unlike general proteomics services, we offer specialized functional assays and are able to provide a comprehensive, one-stop solution from gene expression to functional analysis, giving you a complete picture of P2RX4 activity.

What are the key applications of this analysis in drug discovery?

P2RX4 analysis is essential for identifying and validating new targets for pain, assessing the potency and selectivity of drug candidates, and understanding the mechanism of action of small molecules and biologics. It is a critical tool for preclinical drug development.

Can you customize the analysis to fit my specific research needs?

Absolutely. Our service is highly flexible. We can tailor the analysis to focus on specific aspects of P2RX4, such as post-translational modifications, protein-protein interactions, or specific cell types. We encourage you to schedule a consultation to discuss your project in detail.

What precautions should I take when preparing and shipping my samples?

To ensure the integrity of your samples, it is crucial to follow our detailed shipping guidelines. This typically involves using dry ice for frozen samples, a durable container, and securely sealing all materials. Our client support team will provide you with a comprehensive protocol upon project initiation.

P2RX4 Analysis Service - Creative Biolabs

Are you currently facing challenges in identifying new targets for pain management, or struggling with the complexity of drug candidate validation in neurological disorders? Our P2RX4 analysis service helps you accelerate your drug discovery and diagnostic projects through advanced purinergic receptor profiling, enabling the development of highly specific and effective treatments for chronic pain. We provide comprehensive insights into receptor function and expression, allowing you to move from discovery to validation with confidence.

Introduction of P2RX4

P2RX4 is a vital member of the P2X receptor family, functioning as a ligand-gated ion channel specifically activated by extracellular adenosine triphosphate (ATP). It plays multifaceted roles in physiological processes, including neurotransmission, immune modulation, and pain signaling pathways. Notably, its dysregulation has been linked to diverse pathological conditions such as cancer progression, where it promotes tumor survival and metastasis, autoimmune diseases characterized by aberrant immune responses, and chronic neuropathic pain. Owing to its pivotal involvement in these critical biological and disease mechanisms, P2RX4 has emerged as a highly promising target, attracting significant interest for the development of novel interventions to combat these challenging conditions.

Uncomplicated Workflow

01Sample Preparation and Submission

Our team provides detailed instructions to ensure your biological samples are correctly prepared and shipped, preserving their integrity. We perform an initial quality control check upon arrival to confirm suitability.

02Expression and Functional Analysis

We use quantitative PCR (qPCR) to measure P2RX4 gene expression and Western Blot (WB) to determine protein levels. Functional assays, such as calcium influx or membrane potential assays, are then performed to assess receptor activity in response to ATP and other agonists.

03Ligand Binding and Antagonist Profiling

We screen potential drug candidates for their ability to bind to the P2RX4 receptor and modulate its activity. This step identifies and validates lead compounds, providing critical data on their potency and selectivity.

04Data Acquisition and Processing

Using high-resolution mass spectrometry and other advanced technologies, we collect comprehensive data on receptor abundance, modifications, and interaction partners. This data is rigorously analyzed using state-of-the-art bioinformatics tools.

05Comprehensive Reporting

The final stage involves compiling all data, analysis, and conclusions into a detailed, easy-to-understand report. This report is delivered securely to you, providing actionable insights for your project.

Why Choose Us?

We leverage deep scientific knowledge, ensuring the highest standards of data accuracy and reliability. Our services are powered by state-of-the-art technologies, including high-throughput screening and mass spectrometry, to provide unparalleled resolution and efficiency. We deliver precise data on P2RX4 expression, function, and modulation, directly addressing key project challenges. Our service is designed to support the identification of novel drug candidates, validate targets, and provide crucial biomarker insights for clinical development. We provide tangible, high-quality data to help you make informed decisions and advance your research with confidence.

Applications

Revolutionizing Cancer Biomarker Discovery

P2RX4 is a groundbreaking biomarker in pan-cancer research, showing overexpression in aggressive tumors like bladder, breast, and glioblastoma. Elevated levels correlate with poorer survival, especially in glioblastoma and low-grade glioma, making it vital for early diagnosis and personalized treatment.

Precision Tumor Immune Modulation

P2RX4 shapes tumor immune environments by inversely affecting CD8⁺ T cell infiltration in breast cancer and thymoma, revealing a mechanism for immune evasion. Targeting P2RX4 offers new ways to enhance immunotherapy and restore anti-tumor defenses.

Validating P2RX4 as a Therapeutic Target

Functional studies confirm P2RX4's oncogenic role across cancers. Knockdown in glioblastoma and prostate cancer reduces proliferation, migration, and invasion while increasing apoptosis. It regulates autophagy and protein trafficking, critical for tumor survival under stress, making it a promising drug target.

Pioneering Autoimmune Disease Intervention

Beyond cancer, P2RX4 sustains plasma cell survival in bone marrow via ATP-PANX3 signaling, preventing apoptosis. Targeting P2RX4 could deplete pathogenic plasma cells, reducing autoantibodies in lupus and rheumatoid arthritis, offering new avenues.

Unraveling Drug Resistance Mechanisms

P2RX4 drives adaptive resistance in solid tumors like colorectal cancer. ATP from dying cells activates P2RX4 in survivors, causing ROS-mediated DNA damage and mTOR dependency. Co-targeting P2RX4 and mTOR enhances oxidative stress, overcoming resistance and improving treatment outcomes.

FAQs

Related Services

Quantitative Proteomics

Gain a complete understanding of protein expression profiles and identify novel P2RX4 interaction partners in your samples.

Post-Translational Modification Analysis

Investigate how phosphorylation, ubiquitination, or other modifications affect P2RX4 function and signaling.

Ready to advance your project? Our team of scientific specialists is available to answer your questions and help you design a study that meets your specific research goals.