Small RNAs are defined as non-coding RNA (ncRNA) molecules less than 200 nt in length, which frequently play pivotal roles in gene silencing and regulation of gene expression. At Creative Biolabs, we harness cutting-edge NGS technology along with rigorous data analysis procedures to offer premium small RNA sequencing services, encompassing the discovery, expression profiling, and mutation characterization of novel small RNAs.
Overview of Small RNA
Small RNAs encompass a wide spectrum of molecules, ranging from small interfering RNA (siRNA) and microRNA (miRNA) to piwi-interacting RNA (piRNA), along with small nucleolar RNA (snoRNA) and small nuclear RNA (snRNA). Generally, these small RNAs are generated through the fragmentation of longer RNA sequences, facilitated by specific enzymes and associated proteins. Notably, the composition of small RNA populations can vary extensively across different tissue types and species. Extensive research has demonstrated that small RNAs play crucial roles in numerous biological processes, spanning development, cell proliferation, and apoptosis.
Fig.1 Main modes of action of prokaryotic sRNAs.1
Featured Services
Small RNA sequencing, leveraging the advanced capabilities of NGS technology, represents a pivotal approach for isolating and acquiring insights into non-coding RNA molecules. At Creative Biolabs, our small RNA sequencing enables the discrimination of small RNAs from their larger counterparts within RNA families. Furthermore, it serves as a tool for uncovering novel small RNAs, examining differential expression profiles across the entire small RNA spectrum, and delineating variations with single-base precision. Our featured sequencing services help you gain a deeper understanding of the role of small RNAs in cellular function and gene expression dynamics.
Detailed Service Process
Advantages
- Comprehensive Coverage
Captures all classes of small RNAs, including siRNAs, microRNAs, piRNAs, and others, revealing the full complexity of regulatory networks.
- High-Throughput
A single sequencing run can generate over 5 million sequence reads.
- High Sensitivity
High-throughput sequencing of small RNAs boasts inherent high sensitivity, capable of detecting scarce ncRNA molecules.
- Excellent Reproducibility
In-depth sequencing ensures the randomness of samples, providing excellent reproducibility without the need for repeated experiments.
- Customizable
Tailor library preparation and sequencing strategies to match your unique research objectives.
Scientific Research Application
- Disease Mechanisms
Investigate the role of small RNAs in cancer, neurological disorders, and other complex diseases.
- Developmental Biology
Study small RNA-mediated regulatory changes during embryonic development and differentiation.
- Crop Enhancement
Pinpointing small RNA targets to bolster crop resilience, elevate yields, and enrich nutritional quality.
- Pharmaceutical Development
Discover novel drug targets and biomarkers by profiling small RNA changes in response to therapeutic interventions.
Contact us now to discuss your project and explore how our small RNA sequencing services can propel your research to new heights.
Reference
- González Plaza, Juan José. "Small RNAs as fundamental players in the transference of information during bacterial infectious diseases." Frontiers in Molecular Biosciences 7 (2020): 101. Distributed under Open Access license CC BY 4.0, without modification.
For Research Use Only.
